Wada lab - Publication

業績

2025

"Solution-Phase Synthesis of Boranophosphate and Boranophosphate/Phosphorothioate/Phosphate Chimeric Oligonucleotides via the H-Boranophosphonate Method "
Yuhei Takahashi, Ituki Kato, Kazuki Sato, Takeshi Wada
J. Org. Chem. (2025)
https://doi.org/10.1021/acs.joc.5c00583

"Stereoselective Synthesis of Nucleotide Analog Prodrugs (Protides) via an Oxazaphospholidine Method "
Monta Nakamura, Kiyoshi Kakuta, Kazuki Sato, Takeshi Wada
J. Org. Chem. (2025)
doi.org/10.1021/acs.joc.5c00240

" Stereocontrolled synthesis and nuclear magnetic resonance analysis of boranophosphate/phosphate chimeric oligonucleotides"
Kiyoshi Kakuta, Taiichi Sakamoto, Hajime Sato, Kazuki Sato, Takeshi Wada
R. Soc. Open Sci 12, 241797(2025)
DOI:10.1098/rsos.241797

"Preparation of Boranophosphate DNA via the Boranophosphotriester Method and Synthesis of P-modified DNAs from Boranophosphate DNA via an Acyl Phosphite Intermediate"
Yuhei Takahashi, Kazuki Sato, Takeshi Wada
Current Protocols e70117(2025)
DOI:10.1002/cpz1.70117

2024

"DNA/RNA heteroduplex technology with cationic oligopeptide reduces class-related adverse effects of nucleic acid drugs"
Masahiro Ohara, Tetsuya Nagata, Rintaro Iwata Hara, Kie Yoshida-Tanaka, Nozomi Toide, Kazunori Takagi, Kazuki Sato, Tomoya Takenaka, Masanori Nakakariya, Kenichi Miyata, Yusuke Maeda, Kazuko Toh, Takeshi Wada, Takanori Yokota
Mol. Ther. Nucleic. Acids 35, 102289 (2024)
DOI:10.1016/j.omtn.2024.102289

"α-Selective Solid-Phase Synthesis of Glycosyl PhosphateRepeating Structure Via the Phosphoramidite Method"
Kazuki Kawato, Kazuki Sato, Takeshi Wada
Chem. Eur. J. e202401226 (2024)
DOI:10.1002/chem.202401226

"Development of cationic oligodiaminogalactoses specifically binding to duplex RNA, but not to duplex DNA"
Hidetaka Torigoe, Sumire Nakayama, Tomomi Shiraishi, Kazuki Sato, Rintaro Iwata Hara, Takeshi Wada
Thermochimica Acta 738, 179789(2024)
DOI:10.1016/j.tca.2024.179789

"ICLAMP: a novel technique to explore adenosine deamination via inosine chemical labeling and affinity molecular purification"
Yuxi Yang, Koki Nakayama, Shunpei Okada, Kazuki Sato, Takeshi Wada, Yuriko Sakaguchi, Ayaka Murayama, Tsutomu Suzuki, Masayuki Sakurai
FEBS Lett. 598, 1080-1093(2024)
DOI:10.1002/1873-3468.14854

2023

"Analysis of Acetylated Hyaluronic Acid in Moisturizing Lotion and Milk Lotion by HPLC with Fluorescence Detection"
Momo Shimekake, Masahiro Komeno, Manawo Taguchi, Shoo Katsumoto, Yuna Tanda, Kazuki Sato, Takeshi Wada, Toshihiko Toida, Kyohei Higashi
Chem. Pharm. Bull 71, 616-619(2023)
DOI:10.1248/cpb.c23-00094

"Development of a quick preparation method for the analysis of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in human urine by phenylboronic-acid solid-phase extraction"
Ayumu Ishii, Kazuki Sato, Kosuke Kusakabe, Noriyuki Kato, Takeshi Wada
J. Pharm. Biomed. Anal. 234, 115556(2023)
DOI:10.1016/j.jpba.2023.115556

"Internucleotidic bond formation using H-phosphonamidate derivatives and acidic activators"
Taiki Tsurusaki, Kazuki Sato, Takeshi Wada
RSC Adv. 13, 31674-31686(2023)
DOI:10.1039/D3RA06193K

" Solid-Phase Synthesis of Oligodeoxynucleotides Using Nucleobase-N-Unprotected Oxazaphospholidine Derivatives Bearing a Long Alkyl Chain"
Kiyoshi Kakuta, Ryouta Kasahara, Kazuki Sato, Takeshi Wada
Org. Biomol. Chem. 21, 7580-7592(2023)
DOI:10.1039/D3OB01255G

"Convergent synthesis of phosphorodiamidate morpholino oligonucleotides (PMOs) by the H-phosphonate approach"
Taiki Tsurusaki, Kazuki Sato, Hiroki Imai, Kunihiro Hirai, Daisuke Takahashi, Takeshi Wada
Sci Rep 13, 12576(2023)
DOI:10.1038/s41598-023-38698-2

"Fucosylated heparan sulfate from the midgut gland of Patinopecten yessoensis"
Shoichi Onishi, Kento Shionoya, Kazuki Sato, Ayumu Mubuchi, Shiori Maruyama, Tadaaki Nakajima, Masahiro Komeno, Shinji Miyata, Kazumi Yoshizawa, Takeshi Wada, Robert J. Linhardt, Toshihiko Toida, Kyohei Higashi
Carbohydrate Polymers. 313, 120847(2023)
DOI:10.1016/j.carbpol.2023.120847

"Synthesis of P-Modified DNA from Boranophosphate DNA as a Precursor via Acyl Phosphite Intermediates. "
Yuhei Takahashi, Kiyoshi Kakuta, Yukichi Namioka, Ayumi Igarashi, Taiichi Sakamoto, Rintaro Iwata Hara, Kazuki Sato, Takeshi Wada.
J. Org. Chem. 88, 10617–10631(2023)
DOI:10.1021/acs.joc.3c00659

"Solid-Phase Synthesis of Glycosyl Phosphate Repeating Units via Glycosyl Boranophosphates as Stable Intermediates"
Kazuki Sato, Kazumasa Muramoto, Tomoya Hagio, Rintaro Iwata Hara, Takeshi Wada
Org. Lett. 25, 3927−3931 (2023)
DOI:10.1021/acs.orglett.3c01293

"Solid-phase synthesis and properties of stereocontrolled boranophosphate/phosphate and phosphorothioate/phosphate chimeric oligouridylates"
Kazuki Sato, Yohei Nukaga and Takeshi Wada
R. Soc. Open Sci. 10, 230095(2023)
DOI:10.1098/rsos.230095

"Properties of artificial cationic oligodiaminosaccharides and oligopeptides that bind to A-type oligonucleotide duplexes"
Tomomi Shiraishi, Kazuki Sato, Rintaro Iwata Hara and Takeshi Wada
Nucleosides, Nucleotides & Nucleic Acids. 42, 619-636 (2023)
DOI:10.1080/15257770

"Development of a new synthetic method for oligodeoxynucleotides using 3ʹ-H-phosphonamidate derivatives"
Taiki Tsurusaki, Kazuki Sato and Takeshi Wada
Org. Biomol. Chem. 21, 2486-2492 (2023)
DOI:10.1039/D2OB02292C

2022

"Synthesis and properties of oligodiaminogalactoses that bind to A-type oligonucleotide duplexes"
Tomomi Shiraishi, Kazuki Sato, Rintaro Iwata Iwata Hara and Takeshi Wada
Org. Biomol. Chem. 20, 8243-8258 (2022)
DOI:10.1039/D2OB01575G

"Solid-phase synthesis of N-trichloroacetyl mannosamine 1-phosphate repeating units mimicking capsular polysaccharide derived from Neisseria meningitidis serotype A"
Kazuki Sato, Akie Chiba, Tomomi Shiraishi, Yuki Ogawa, Rintaro Iwata Hara, Takeshi Wada
Carbohydr. Res. 518, 108585 (2022)
DOI:10.1016/j.carres.2022.108585

"Identification and Quantitative Analysis of 2-Fluoro Methamphetamine and its Metabolites in Human Urine"
Ayumu Ishii, Kazuki Sato, Kosuke Kusakabe, Noriyuki Kato, Takeshi Wada
J. Anal. Toxicol. 47, 59–65 (2022)
DOI: 10.1093/jat/bkac026

"Comprehensive analysis of chondroitin sulfate and aggrecan in the head cartilage of bony fishes: Identification of proteoglycans in the head cartilage of sturgeon"
Shionoya, K., Suzuki, T., Takada M., Sato, K., Onishi, S., Dohmae, N., Nishino, K., Wada, T., Linhardt, R. J., Toida, T., Higashi, K.
Int. J. Biol. Macromol. 208, 333-342 (2022)
DOI: 10.1016/j.ijbiomac.2022.03.125

"Solid-Phase Synthesis of Boranophosphate/Phosphorothioate/Phosphate Chimeric Oligonucleotides and Their Potential as Antisense Oligonucleotides"
Takahashi, Y., Sato, K., Wada, T.
J. Org. Chem. 87, 3895-3909 (2022)
DOI: 10.1021/acs.joc.1c01812 Selected as the front cover.

2021

"Solution-phase synthesis of oligodeoxyribonucleotides using the H-phosphonate method with N-unprotected 5'-phosphite monomers"
Matsuda, H., Yoshida, E., Shinoda, T., Sato, K., Hara, R. I., Wada, T.
RSC Adv. 11, 38094-38107 (2021)

"Solid-phase synthesis oh oligouridine boranophosphates using the H-boranophosphonate method with 2'-O-(2-cyanoethoxymethyl) protection"
Matsuda, H., Ito, H., Nukaga, Y., Uehara, S., Sato, K., Hara, R. I., Wada, T.
Tetrahedron Lett. 87, 153526 (2021)

"Cholesterol-functionalized DNA/RNA heteroduplexes cross the blood-brain barrier and knock down genes in the rodent CNS"
Nagata, T., Dwyer, C. A., Yoshida, K. T., Ihara, K., Ohyagi, M., Kaburagi, M., Miyata, H., Ebihara, S., Yoshioka, K., Ishii, T., Miyata, K., Miyata, K., Powers, B., Igari, T., Yamamoto, S., Arimura, N., Hirabayashi, H., Uchihara, T., Hara, R. I., Wada, T., Bennett, F. C., Seth, P. P., Rigo, F., Yokota, T.
Nat. Biotechnol. 39, 1529-1536 (2021)

"Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide"
Hara, R. I., Wada, T.
Org. Biomol. Chem. 19, 8675-6870 (2021)

"Solid-Phase Stereocontrolled Synthesis of Oligomeric P-Modified Glycosyl Phosphate Derivatives Using the Oxasaphospholidine Method"
Sato, K., Hagio, T., Sano, M., Muramoto, K.,Yaoita, A., Noro, M., Hara, R. I., Wada, T.
ACS Omega 6, 20026-20041 (2021)

"Synthesis of thioamides from thiocarboxylic acids using phosphonium-type condensing reagents"
Suzuki, A., Takagi, K., Sato, K., Wada, T.
Tetrahedron Lett. 75, 153179 (2021)

"糖-1-リン酸繰り返し構造およびその化学修飾アナログの合成"
Hara, R. I., Sato, K., Wada, T.
有機合成化学協会誌 79, 558-569 (2021)

2020

"Synthesis and properties of DNA oligomers containing stereopure phosphorothioate linkages and C-5 modified deoxyuridine derivatives"
Hara, R. I., Yoshino, R., Nukaga, Y., Maeda, Y., Sato, K., Wada, T.
RSC Adv. 10, 34006-34013 (2020)

"An artificial cationic oligosaccharide combined with phosphorothioate linkages strongly improves siRNA stability"
Irie, A., Sato, K., Hara, R. I., Wada, T., Shibasaki, F.
Sci. Rep. 10, 14845(2020)

"Enhanced Immunostimulatory Activity of CpG Oligonucleotide by the Combination of Mannose Modification and Incorporation into Nanostructured DNA"
LIao, W., Akahira, S., Hara, R. I. Wada, T., Kusamori, K., Takakura, Y., Nishikawa, M.
Biol.Pharm. Bull. 43, 11881-1195 (2020).

"ボラノホスフェート核酸の効率的な合成法の開発"
Sato, K., Wada, T.
有機合成化学協会誌 78, 421-434 (2020).

"Studies on the phase I metabolites of the new designer drug 1-(2,3-dihydro-1H-inden-5-yl)-2-(pyrrolidine-1-yl)butan-1-one (5-PPDI) in human urine"
Ishii, A., Kusakabe, K.,Kato, N., Sasaki, S., Tsujikawa, K., Wada, T.
Forensic. Sci. Int. 310, 110214 (2020).

2019

"Solid-Phase Synthesis of Phosphate/Boranophosphate Chimeric DNAs Using the H-Phosphonate-H-Boranophosphonate Method "
Sato, K.,Imai, H., Syuto, T., Hara, R.I., Wada, T.
J. Org. Chem. 84, 15032-15041 (2019).

"Highly efficient silencing of microRNA by heteroduplex oligonucleotides "
Yoshioka, K.,Kunieda, T., Asami, Y., Guo, H., Miyata, H., Yoshida-Tanaka, K., Sujino, Y., Piao, W., Kuwahara, H., Nishina, K., Hara, R. I., Nagata, T., Wada, T., Obika, S., Yokota, T.
Nucleic Acids Res. 47, 7321-7332 (2019).

"Stereocontrolled Synthesis of Boranophosphate DNA by an Oxazaphospholidine Approach and Evaluation of Its Properties "
Hara, R. I., Saito, T., Kogure, T., Hamamura, Y., Uchiyama, N., Nukaga, Y., Iwamoto, N., Wada, T.
J. Org. Chem. 84, 7971-7938 (2019).

"Artificial cationic peptides that increase nuclease resistance of siRNA without disturbing RNAi activity "
Maeda, Y., Hara, R. I., Nishina, K., Yoshida-Tanaka, K., Sakamoto, T., Yokota, T., Wada, T.
Nucleos. Nucleot. Nucl. Acids. 38, 338-348 (2019).

2018

"Enhancement in RNase H activity of a DNA/RNA hybrid duplex using artificial cationic oligopeptides "
Hara, R. I., Maeda, Y., Fujimaki, H., Wada, T.
Chem. Commun. 54, 8526-8529 (2018).

"Solid-Phase Synthesis of Fluorinated Analogues of Glycosyl 1-Phosphate Repeating Structures from Leishmania using the Phosphoramidite Method "
Hara, R. I., Yaoita, A., Takeda, K., Ueki, H., Ishii, A., Imoto, H., Kobayashi, S., Sano, M., Noro, M., Sato, K., Wada, T.
ChemistryOpen. 7, 439-446 (2018). (selected as "Front Cover")

"Artificial cationic oligosaccharides for heteroduplex oligonucleotide-type drugs "
Hara, R. I., Hisada, Y., Maeda, Y., Yokota, T., Wada, T.
Sci. Rep. 8, 4323 (2018).

"Solid-Phase Synthesis of Oligopeptides Containing Sterically Hindered Amino Acids on Nonswellable Resin Using 3-Nitro-1,2,4-triazol-1-yl-tris(pyrrolidin-1-yl)phosphonium Hexafluorophosphate (PyNTP) as the Condensing Reagent "
Hara, R. I., Mitsuhashi, Y., Saito, K., Maeda, Y., Wada, T.
ACS Comb. Sci. 20, 132-136 (2018).

2017

"Synthesis of 2′-O-monohaloethoxymethyl-modified RNAs and their duplex formation ability "
Hara, R. I., Kageyama, M., Arai, K., Uchiyama, N., Wada, T.
RSC Adv. 7, 41297-41303 (2017).

"Synthesis and properties of 2-deoxy-2-fluoromannosyl phosphate derivatives "
Hara, R. I., Kobayashi, S., Noro, M., Sato, K., Wada, T.
Tetrahedron 73, 4560-4565 (2017).

"Stereocontrolled Synthesis of P-Modified N-Acetylmannosamine-α-1-phosphate Analogs "
Hara, R. I., Ogawa, Y., Noro, M., Wada, T.
Chem. Lett. 46, 1051-1053 (2017).

"Double-stranded RNA-binding artificial cationic oligosaccharides stabilizing siRNAs with a low N/P ratio "
Hara, R. I., Maeda, Y., Sakamoto, T., Wada, T.
Org. Biomol. Chem. 15, 1710-1717 (2017).

2016

"One-pot conversion reactions of glycosyl boranophosphates into glycosyl phosphate derivatives via acyl phosphite intermediates "
Sato, K., Wada, T.
Org. Biomol. Chem. 14, 11092-11095 (2016).

"Stereocontrolled Solid-Phase Synthesis of Phosphate/Phosphorothioate (PO/PS) Chimeric Oligodeoxyribonucleotides on an Automated Synthesizer Using an Oxazaphospholidine-Phosphoramidite Method "
Nukaga, Y., Oka, N., Wada, T.
J. Org. Chem. 81, 2753-2762 (2016).

2015

"Synthesis of oligodiaminomannoses and analysis of their RNA duplex binding properties and their potential application as siRNA-based drugs "
Iwata, R., Doi, A., Maeda, Y., Wada, T.
Org. Biomol. Chem. 13, 9504-9515 (2015).

"Enhancement of affinity of 2´-O-Me-oligonucleotides for complementary RNA by incorporating a stereoregulated boranophosphate backbone "
Nukaga, Y., Takemura, T., Iwamoto, N., Oka, N., Wada, T.
RSC Adv. 5, 2392-2395 (2015).

"Synthesis and properties of vitamin E analog-conjugated neomycin for delivery of RNAi drugs to liver cells "
Iwata, R., Nakayama, F., Hirochi, S., Sato, K., Piao, W., Nishina, K., Yokota, T., Wada, T.
Bioorg. Med. Chem. Lett. 25, 815-819 (2015).

"Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol "
Nishina, T., Numata, J., Nishina, K., Yoshida-Tanaka, K., Nitta, K., Piao, W., Iwata, R., Ito, S., Kuwahara, H., Wada, T., Mizusawa, H., Yokota, T.
Mol. Ther. Nucleic Acids 4, e220 (2015).

2014

"Solid-phase Synthesis of P-Boronated Oligonucleotides by the H-Boranophosphonate Method "
Uehara, S., Hiura, S., Higashida, R., Oka, N., Wada, T.
J. Org. Chem. 79, 3465-3472 (2014).

"Synthesis and properties of double-stranded RNA-bindable oligodiaminogalactose derivatives conjugated with vitamin E "
Iwata, R.; Nishina, K.; Yokota, T.; Wada, T.
Bioorg. Med. Chem. 22, 1394-1403 (2014).

"3-Nitro-1,2,4-triazol-1-yl-tris(pyrrolidin-1-yl)phosphonium hexafluorophosphate (PyNTP) as a condensing reagent for solid-phase peptide synthesis "
Saito, K.; Wada, T.
Tetrahedron Lett. 55, 1991-1993 (2014).

2013

"Stereoselective Synthesis of P-Modified α-Glycosyl Phosphates by the Oxazaphospholidine Approach "
Noro, M.; Fujita, S.; Wada, T.
Org. Lett. 23, 5948-5951 (2013).

"Synthesis and properties of cationic oligopeptides with different side chain lengths that bind to RNA duplexes "
Maeda, Y., Iwata, R., Wada, T.
Bioorg. Med. Chem. 21, 1717-1723 (2013).

"Synthesis of Novel Oligo Cationicpeptides Binding to RNA Duplexes "
Maeda, Y., Iwata, R., Wada, T.
Peptide Science 2012, 23-24 (2013).

"Stereocontrolled synthesis of di phosphorothioates using a fluorous tag "
Oka, N., Murakami, R., Kondo, T., Wada, T.
J. Fluor. Chem. 150, 85-91 (2013).

"Glycosylation of alcohols using glycosyl boranophosphates as glycosyl donors "
Tatsumi, S., Matsumura, F., Oka, N., Wada, T.
Tetrahedron Lett. 54, 3731-3734 (2013).

"A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases "
Ohmuro-Matsuyama, Y., Nakano, K., Kimura, A., Ayabe, K., Ihara, M., Wada, T., Ueda, H.
Anal. Chem. 85, 7935-7940 (2013).

2012

"Stereocontrolled Solid-Phase Synthesis of Phosphorothioate Oligoribonucleotides Using 2’-O-(2-Cyanoethoxymethyl)-Nucleoside 3’-O-Oxazaphospholidine Monomers "
Nukaga, Y.; Yamada, K; Ogata, T.; Oka, N.; Wada, T.
J. Org. Chem. 77, 7913-7922　(2012)

"Stereocontrolled synthesis of oligodeoxyribonucleoside boranophosphates by an oxazaphospholidine approach using acid-labile N-protecting groups "
Iwamoto, N., Oka, N., Wada, T.
Tetrahedron Lett. 53, 4361-4364 (2012).

"Synthesis of nucleoside 5´-boranophosphorothioate derivatives using an H-boranophosphonate monoester as a precursor "
Oka, N., Takayama, Y., Ando, K., Wada, T.
Bioorg. Med. Chem. Lett. 22, 4571-4574 (2012).

"Improved method for the solid-phase synthesis of oligoribonucleotide 5´-triphosphates "
Nagata, S., Takagaki, K., Wada, T.
Chem. Pharm. Bull. 60, 1212-1215 (2012).

"Recent Progress in the Synthesis of Glycosyl Phosphate Derivatives "
Oka, N.; Sato, K.; Wada, T.
Trends Glycosci. Glycotechnol. 24, 152-168 (2012).

"Synthesis of Novel Oligocationic Peptides which Bind to A-Type Nucleic Acid Duplexes "
Maeda, Y., Iwata, R., Wada, T.
Peptide Science 2011 231-232 (2012).

2011

"Synthesis and properties of novel 2´-O-alkoxymethyl-modified nucleic acids "
Arai, K., Uchiyama, N., Wada, T.
Bioorg. Med. Chem. Lett. 21, 6285-6287 (2011).

"Stereocontrolled synthesis of oligonucleotide analogs containing chiral internucleotidic phosphorus atoms "
Oka, N., Wada, T.
Chem. Soc. Rev. 40, 5829-5843 (2011).

"Synthesis of Oligodiaminosaccharides Having α-Glycoside Bonds and Their Interactions with Oligonucleotide Duplexes "
Iwata, R., Sudo, M., Nagafuji, K., Wada, T.
J. Org. Chem. 76, 5895-5906 (2011).

"Trimethylsilyl Trifluoromethanesulfonate-promoted Reductive 2′-O-arylmethylation of Ribonucleoside Derivatives "
Uchiyama, N., Ogata, T., Oka, N., Wada, T.
Nucleos. Nucleot. Nucl. Acids 30, 446-456 (2011).

"Synthesis of Oligo(α-D-glycosyl phosphate) Derivatives by a Phosphoramidite Method via Boranophosphate Intermediates "
Fujita, S., Oka, N., Matsumura, F., Wada, T.
J. Org. Chem. 76, 2648-2659 (2011).

2010

"Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea "
Ikeuchi, Y., Kimura, S., Numata, T., Nakamura, D., Yokogawa, T., Ogata, T., Wada, T., Suzuki, T., Suzuki, T.
Nat. Chem. Biol. 6, 277-282 (2010).

"1,2-trans-selective synthesis of Glycosyl Boranophosphates and their utility as building blocks for the synthesis of phosphodiester-linked disaccharides "
Sato, K., Oka, N., Fujita, S., Matsumura, F., Wada, T.
J. Org. Chem. 75, 2147-2156 (2010).

"Synthesis and biological activity of artificial mRNA prepared with novel phosphorylating reagents "
Nagata, S., Hamasaki, T., Uetake, K., Masuda, H., Takagaki, K., Oka, N., Wada, T., Ohgi, T., Yano, J.
Nucleic Acids Res.38, 7845-7857 (2010).

"Synthesis, gene-silencing activity and nuclease resistance of 3'-3'-linked double short hairpin RNA "
Masuda H., Watanabe N., Naruoka H., Nagata S., Takagaki K., Wada T., Yano J
Bioorg. Med. Chem.18, 8277-8283 (2010).

"Rapid glycosylation under extremely mild acidic conditions. Use of ammonium salts to activate glycosyl phosphites via P-protonation "
Matsumura, F., Tatsumi, S., Oka, N., Wada, T.
Carbohyd. Res. 345, 1211-1215 (2010).

"Solid-phase synthesis of oligodeoxyribonucleotides without base protection utilizing O-selective reaction of oxazaphospholidine derivatives "
Oka, N., Maizuru, Y., Shimizu, M., Wada, T.
Nucleos. Nucleot. Nucl. Acids 29, 144-154 (2010).

2009

"Stereocontrolled Solid-phase Synthesis of Oligonucleoside H-phosphonates by an Oxazaphospholidine Approach "
Iwamoto, N., Oka, N., Sato, T., Wada, T.
Angew. Chem. Int. Ed. 48, 496-499 (2009).

"Stereocontrolled Synthesis of Oligoribonucleoside Phosphorothioates by an Oxazaphospholidine Approach "
Oka, N., Kondo, T., Fujiwara, S., Maizuru, Y., Wada, T.
Org. Lett. 11, 967-970 (2009).

"Chemical Synthesis and Properties of 5-Taurinomethyluridine and 5-Taurinomethyl-2-thiouridine "
Ogata, T., Shimazaki, T., Umemoto, T., Kurata, S., Ohtsuki, T., Suzuki, T., Wada, T.
J. Org. Chem. 74, 2585-2588 (2009).

"Nucleoside H-Boranophosphonates: a New Class of Boron-Containing Nucleotide Analogues "
Higashida, R., Oka, N., Kawanaka, T., Wada, T.
Chem. Commun. 2466-2468 (2009).

"Synthesis and Antiviral Evaluation of 5-(1,2,3-Triazol-1-ylmethyl)uridine Derivatives "
Abdel-Rahman, A. A.-H., Wada, T.
Z. Naturforsch. C. 64, 163-166 (2009).

"Stereocontrolled synthesis of tertiary silanes via optically pure 1,3,2-oxazasilolidine derivatives "
Oka, N., Nakamura, M., Soeda, N., Wada, T.
J. Organomet. Chem. 694, 2171-2178 (2009).

"Stereocontrolled synthesis of oligodeoxyribonucleoside boranophosphates via stereodefined H-phosphonate intermediates "
Iwamoto, N., Oka, N., Wada, T.
Nucleic Acids Symp. Ser. 53, 9-10 (2009).

"Chemical synthesis of nucleoside H-boranophopsphonates and their application as precursors of P-modified nucleotide analogues "
Oka, N., Higashida, R., Takayama, Y., Ando, K.,Wada, T.
Nucleic Acids Symp. Ser.53, 111-112 (2009).

"Stabilization of A-type nucleic acid duplexes by novel oligodiaminosaccharides "
Iwata, R., Sudo, M., Nagafuji, K., Wada, T.
Nucleic Acids Symp. Ser. 53, 113-114(2009).

2008

"Solid-phase Synthesis of Stereoregular Oligodeoxyribonucleoside Phosphorothioates Using Bicyclic Oxazaphospholidine Derivatives as Monomer Units "
Oka, N., Yamamoto, M., Sato, T., Wada, T.
J. Am. Chem. Soc. 130, 16031-16037 (2008).

"Synthesis of Glycosyl Boranophosphates and Their Applications as Precursors of Glycosyl Phosphate Analogues "
Matsumura, F., Oka, N., Wada, T.
Org. Lett. 8, 1557-1560 (2008).

"Stereoselective Synthesis of α-Glycosyl Phosphites and Phosphoramidites via O-Selective Glycosylation of H-Phosphonate Derivatives "
Matsumura, F., Oka, N., Wada, T.
Org. Lett. 10, 5297-5300 (2008).

"Chemical synthesis of diastereomeric diadenosine boranophosphates (ApbA) from 2'-O-(2-cyanoethoxymethyl)adenosine by the boranophosphotriester method"
Enya, Y., Nagata, S., Masutomi, Y., Kitagawa, H., Takagaki, K., Oka, N., Wada, T., Ohgi, T., Yano, J.
Bioorg. Med. Chem. 16, 9154-9160 (2008).

"Solid-phase synthesis of backbone-modefied DNA analogs by the boranophosphotriester method using new protecting groups for nucleobases"
Kawanaka, T., Shimizu, M., Shintani, N., Wada, T.
Bioorg. Med. Chem. Lett. 13, 3783-3786 (2008).

"Modified Uridines with C5-methylene Substituents at the First Position of the tRNA Anticodon Stabilize U・G Wobble Pairing during Decoding"
Kurata, S., Weixlbaumer, A., Ohtsuki, T., Shimazaki, T., Wada, T., Kirino, Y., Takai, K., Watanabe, K., Ramakrishnan, V., Suzuki, T.
J. Biol. Chem. 283, 18801-18811 (2008).

"Stereocontrolled synthesis of backbone-modified oligonucleotides via diastereopure H-phosphonate intermediates"
Iwamoto, N., Oka, N., Wada, T.
Nucleic Acids Symp. Ser. 52, 333-334 (2008).

"Chemical synthesis of RNA including 5-taurinomethyluridine and 5-taurinomethyl-2-thiouridine"
Ogata, T., Wada, T.
Nucleic Acids Symp. Ser. 52, 323-324 (2008).

"Stereocontrolled synthesis of oligonucleoside phosphorothioates and PO/PS-chimeric oligonucleotides by using oxazaphospholidine derivatives"
Oka, N., Yamamoto, M., Sato, T., Wada, T.
Nucleic Acids Symp. Ser. 52, 335-336 (2008).

"Towards Fluorous-Phase Synthesis of Nucleoside β-Peptides"
Threlfall, R., Cosstick, R., Wada, T.
Nucleic Acids Symp. Ser. 52, 337-338 (2008).

2007

"Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase"
Watanabe, K., Toh, Y., Suto, K., Shimizu, Y., Oka, N., Wada, T., Tomita, K.
Nature 449, 867-871 (2007).

"Synthesis of Dinucleoside Phosphates and their Analogs by the Boranophosphotriester Method Using Azido-Based Protecting Groups"
Kawanaka, T., Shintani, N., Shimizu, M, Wada, T.
Tetrahedron Lett. 48, 1973-1976 (2007).

"Chemical synthesis and properties of stereoregulated phosphorothioate RNAs"
Wada, T., Kondo, T., Fujiwara, S., Sato, T., Oka, N.
Nucleic Acids Symp. Ser. 51, 119-120 (2007).

"Nucleoside H-boranophosphonates: Synthesis and properties of a new class of nucleotide analogs"
Higashida, R., Kawanaka, T., Oka, N., Wada, T.
Nucleic Acids Symp. Ser. 51, 133-134 (2007).

"Solid-phase synthesis and properties of chiral peptide nucleic acids bearing cationic side chains"
Harada, H., Funayama, S., Shoji, Y., Wada, T.
Nucleic Acids Symp. Ser. 51, 259-260 (2007).

2006

"Solid-phase Synthesis of Oligodeoxyribonucleoside Boranophosphates by the Boranophosphotriester Method"
Shimizu, M. Saigo, K., Wada, T.
J. Org. Chem. 71, 4262-4269 (2006).

"1,3-Dimethyl-2-(3-nitro-1,2,4-triazol-1-yl)-2-pyrrolidin-1-yl-1,3,2-diazaphospholidinium Hexafluorophosphate: a Powerful Condensing Reagent for Phosphate and Phosphonate Esters"
Oka, N., Shimizu, M., Saigo, K., Wada, T.
Tetrahedron 62, 3667-3673 (2006).

"A Novel Linker for Solid-phase Synthesis Cleavable under Neutral Conditions"
Murata, A., Wada, T.
Tetrahedron Lett. 47, 2147-2150 (2006).

"Highly Chemo- and Regioselective Phosphitylation of Unprotected 2'-Deoxyribonucleosides"
Kato, Y., Oka, N., Wada, T.
Tetrahedron Lett. 47, 2501-2505 (2006).

"Synthesis of a Novel Ester Analog of Nucleic Acids Bearing a Serine Backbone"
Murata, A., Wada, T.
Bioorg. Med. Chem. Lett. 16, 2933-2936 (2006).

"Stereoselective Synthesis of Dinucleoside Boranophosphates by an Oxazaphospholidine Method"
Wada, T., Maizuru, Y., Oka, N., Saigo, K.
Bioorg. Med. Chem. Lett. 16, 3111-3114 (2006).

"Stereocontrolled Syntheisis of H-phopsphonate DNA"
Iwamoto, N., Sato, T., Oka, N., Wada, T.
Nucleic Acids Symp. Ser. 50, 159-160 (2006).

"Solid-phase Synthesis of Stereoregulated Oligodeoxyribonucleoside Phosphorothiates"
Oka, N., Yamamoto, M., Sato, T, Wada, T.
Nucleic Acids Symp. Ser. 50, 123-124 (2006).

"Chemical Synthesis of RNA including 5-Taurinomethyluridine"
Ogata, T., Wada, T.
Nucleic Acids Symp. Ser. 50, 9-10 (2006).

"Synthesis of Novel Chiral Diamino Acid-based Polyamide as Analogues of Nucleic Acids"
Wada, T., Tanabe, S., Saigo, K.
Peptide Science 2005 469-470 (2006).

"Synthesis of Chiral Oligopeptides which Recognize Double-Stranded DNAs"
Funayama, S., Shoji, Y., Saigo, K., Wada, T.
Peptide Science 2005 467-468 (2006).

"Solid-phase Synthesis of a Novel Polyester Analog of Nucleic Acids Bearing a Serine Backbone Using a Linker which Cleaved under Neutral Conditions"
Murata, A., Saigo, K., Wada, T.
Peptide Science 2005 109-110 (2006).

2005

"Nitromethane as a Scavenger of Acrylonitrile in the Deprotection of Synthetic Oligonucleotides"
Umemoto, T., Wada, T.
Tetrahedron Lett. 46, 4251-4253 (2005).

"Stereocontrolled Synthesis of Phosphorothioate DNA by the Oxazaphospholidine Approach"
Wada, T.
Front. Org. Chem. 1, 41-61 (2005).

"Stereocontrolled Synthesis of Dinucleoside Boranophosphates by an Oxazaphospholidine Method"
Oka, N., Maizuru, Y., Shimizu, M., Saigo, K., Wada, T.
Nucleic Acids Symp. Ser. 49, 131-132 (2005).

"A Novel Method for the Synthesis of DNA Using 2'-Deoxyribonucleoside 5'-Phosphites as Monomer Units"
Kato, Y., Saigo, K., Wada, T.
Nucleic Acids Symp. Ser. 49, 129-130 (2005).

"A Novel Method for the Synthesis of DNA and its Analogs by the Use of BH₃ as protecting Group for Phosphonic Acid"
Kawanaka, T., Shimizu, M., Saigo, K., Wada, T.
Nucleic Acids Symp. Ser. 49, 27-28 (2005).

2004

"A Novel Method for the Synthesis of Dinucleoside Boranophosphates by a Boranophosohotriester Method"
Shimizu, M., Wada, T., Oka, N., Saigo, K.
J. Org. Chem. 69, 5261-5268 (2004).

"Oligoribonucleotide Synthesis by the Use of 1-(2-Cyanoethoxy)ethyl (CEE) as a 2'-Hydroxy Protecting Group"
Umemoto, T., Wada, T.
Tetrahedron Lett. 45, 9529-9531 (2004).

"BH₃ as a Protecting Group for Phosphonic Acids: A Novel Method for the Synthesis of Dinucleoside H-Phosphonates"
Shimizu, M., Tamura, K., Wada, T., Saigo, K.
Tetrahedron Lett. 45, 371-374 (2004).

"Synthesis of N-(Trimethylsilylethoxycarbonyl)deoxycytidine and Deoxyadenosine Derivatives as Key Intermediates for the DNA Synthesis Using Fluoride Ion-Promoted Deprotection Strategy"
Sekine, M., Tobe, M., Nagayama, T., Wada, T.
Lett. Org. Chem. 1, 179-182 (2004).

"Stereocontrolled Synthesis of Phosphorothioate RNA by the Oxazaphospholidine Approach"
Wada, T., Fujiwara, S., Sato, T., Oka, N., Saigo, K.
Nucleic Acids Symp. Ser. 48, 57-58 (2004).

"Oligoribonucleotide Synthesis by the Use of 1-(2-Cyanoethoxy)ethyl as a 2'-Hydroxy Protecting Group"
Umemoto, T., Wada, T.
Nucleic Acids Symp. Ser. 48, 9-10 (2004).

"RNA Interference (RNAi) and Chemical Synthesis of RNA"
Wada, T.
Bio Industry 21, 17-24 (2004).

2003

"An Oxazaphospholidine Approach for the Stereocontrolled Synthesis of Oligonucleoside Phosphorothioates"
Oka, N, Wada, T., Saigo, K.
J. Am. Chem. Soc. 125, 8307-8317 (2003).

"Synthesis and Properties of Oligodeoxyribonucleotides Possessing N-Phosphoryl Groups."
Moriguchi, T., Wada, T., Sekine, M.
Eur. J. Org. Chem. 2260-2267 (2003).

"Synthesis of TMG-Capped RNA-DNA Chimeric Oligonucleotides"
Sekine, M., Ushioda, M., Wada, T., Seio, K.
Tetrahedron Lett. 44, 1703-1707 (2003).

"Synthesis of Dinucleoside Boranophosphates by a Boranophosphotriester Approach"
Wada, T., Shimizu, M., Oka, N., Saigo, K.
Nucleosides, Nucleotides, Nucleic Acids 22, 1471-1473 (2003).

"Reaction Mechanism for the Sterercontrolled Synthesis of Phosphorothioate DNA by the Oxazaphospholidine Approach"
Wada, T., Oka, M., Saigo, K.
Nucleosides, Nucleotides, Nucleic Acids 22, 1431-1433 (2003).

"Diastereocontrolled Synthesis of Phosphorothioate DNA via an Oxazaphospholidine Approach Using a Novel Class of Activators, Dialkyl(cyanomethyl)ammonium salts"
Oka, N., Wada, T., Saigo, K.
Nucleosides, Nucleotides, Nucleic Acids 22, 1411-1413 (2003).

"Solid-phase Synthesis of a Novel Polyester Analog of NuScleic Acid Bearing Serine Backbone"
Wada, T., Murata, A., Tsuneyama, T., Saigo, K.
Peptide Science 2002 159-160 (2003).

"Synthesis of Chiral Oligopeptides which Recognize Double-stranded DNAs"
Wada, T., Funayama, S., Shoji, Y., Saigo, K.
Peptide Science 2002 181-182 (2003).

"Decoding Property of C5 Uridine Modification at the Wobble Position of tRNA Anticodon"
Kurata, S., Ohtsuki, T., Wada, T., Kirino, Y., Takai, K., Saigo, K., Watanabe, K., Suzuki, T.
Nucleic Acids Res. Suppl. 3, 245-246 (2003).

"Highly Chemo- and Regioselective Phosphitylation of 2'-Deoxyribonucleosides"
Wada, T., Kato, Y. and Saigo, K.
Nucleic Acids Res. Suppl. 3, 65-66 (2003).

"Stereocontrolled Synthesis of Phosphorothioate DNA by an Oxazaphospholidine Approach"
Wada, T., Oka, N. and Saigo, K.
Nucleic Acids Res. Suppl. 3, 109-110 (2003).

2002

"Diastereocontrolled Synthesis of Dinucleoside Phosphorothioates Using a Novel Activators, Dialkyl(cyanomethyl)ammonium Tetrafluoroborates"
Oka, N., Wada, T., Saigo, K.
J. Am. Chem. Soc. 124, 4962-4963 (2002).

"Taurine as a Constituent of Mitochondrial tRNAs: New Insight into the Functions of Taurine and Human Mitochondrial Diseases"
Suzuki, T., Suzuki, T., Wada, T., Saigo, K., Watanabe, K.
EMBO J. 21, 6581-6589 (2002).

"A New Boranophosphorylation Reaction for the Synthesis of Deoxyribonucleoside Boranophosphates"
Wada, T., Shimizu, M., Oka, N. and Saigo, K.
Tetrahedron Lett. 43, 4137-4140 (2002).

"Stereocontrolled Synthesis of Phsphorothioate DNA by the Oxazaphospholidine Approach"
Wada, T., Oka, N., Saigo, K.
Antisense 6, 89-103 (2002).

"Chemical Synthesisi of Novel Taurine-containing Uridine Derivertives"
Wada, T., Shimazaki, T., Nakagawa, S., Ohtsuki, T., Kurata, S., Watanabe, K. and Saigo, K.
Nucleic Acids Res. Suppl. 2, 11-12 (2002).

"Reaction Mechanism for the Stereoselective Internucleotidic Bond Formation by the Oxazaphospholidine Approach"
Wada, T., Oka, N. and Saigo, K.
Nucleic Acids Res. Suppl. 2, 153-154 (2002).

2001

"Synthesis and Hybridization Ability of Oligodeoxyribonucleotides incorporating N-Acyldeoxycytidine Derivatives"
Wada, T., Kobori, A., Kawahara, S., Sekine, M.
Eur. J. Org. Chem. 4583-4593 (2001).

"Synthesis and Conformational Properties of Dinucleoside Triester Derivatives Involving a Cyclonucleotide Bridge Structure"
Sekine, M., Kurasawa, O., Shoda, K., Seio, K., Wada, T.
Eur. J. Org. Chem. 1989-1999 (2001).

"Facile Methods for the Synthesis of 5-Formylcytidine"
Abdel-Rahman, A. A.-H., Wada, T., Saigo, K.
Tetrahedron Lett. 42, 1061-1063 (2001).

"2-(Azidomethyl)benzoyl as a New Protecting group in Nucleosides"
Wada, T., Ohkubo, A., Mochizuki, A., Sekine, M.
Tetrahedron Lett. 42, 1069-1072 (2001).

"Solid-phase Synthesis of a 5'-Terminal TMG-Capped Trinucleotide Block of U1 snRNA "
Kadokura, M., Wada, T., Seio, K., Moriguchi, T., Huber, J., Luhrmann, R., Sekine, M.
Tetrahedron Lett. 42, 8853-8856 (2001).

"Synthesis and Properties of 2-Azidodeoxyadenosine and its Incorporation into Oligodeoxynucleotides"
Wada, T., Mochizuki, A., Higashiya, S., Tsuruoka, H., Kawahara, S., Ishikawa, M., Sekine, M.
Tetrahedron Lett. 42, 9215-9219 (2001).

"Stereoselective Synthesis of Dinucleoside Phosphorothioate Using Enantiopure 1,2-Amino Alcohols as Chral Auxiliaries"
Oka, N., Wada, T., Saigo, K.
Nucleic Acid Res. Suppl. 1, 13-14 (2001).

"Synthesis of a New Type of Artificial Nucleic Acid Derived from Optically Active Serine"
Wada, T., Tsuneyama, T., Saigo, K.
Nucleic Acid Res. Suppl. 1, 187-188 (2001).

"Novel Taurine-containing Uridine Derivatives and Mitochondrial Human Diseases"
Suzuki, T., Suzuki, T., Wada, T., Saigo, K., Watanabe, K.
Nucleic Acid Res. Suppl. 1, 257-258 (2001).

"Total Synthesis of Agrocin 84 and Phosmidosine as Naturally Occurring Nucleotidic Antibiotics Having P-N Bond Linkages"
Sekine, M., Moriguchi, T., Wada, T., Seio, K.
Synth. Org. Chem. Jpn. 59,1109-1120 (2001).

2000

"Synthesis and Properties of Oligodeoxynucleotides Incorporating a Conformationally Rigid Uridine Unit Having a Cyclic Structure at the 5'-Terminal Site"
Sekine, M., Kurasawa, O., Shohda, K., Seio, K., Wada, T.
J. Org. Chem. 65, 3571-3578 (2000).

"Synthesis of 4-Thiouridine, 6-Thioinosine, and 6-Thioguanosine 3',5'-O-Bisphosphates as Donor Molecules for RNA Ligation and Their Application to the Synthesis of Photoactivatable TMG-Capped U1 snRNA Fragments"
Kadokura, M., Wada, T., Seio, K., Sekine, M.
J. Org. Chem. 65, 5104-5113 (2000).

"Synthesis and Properties of Oligonucleotides Having a Phosphorus Chiral Center by Incorporation of Conformationally Rigid 5'-Cyclouridylic Acid Derivatives"
Sekine, M., Kurasawa, O., Shoda, K., Seio., K, Wada, T.
J. Org. Chem. 65, 6515-6524 (2000).

"Synthesis and Conformational Properties of Oligonucleotides Incorporating 2'-O-Phosphorylated Ribonucleotide as Structural Motifs of Pre-tRNA Splicing Intermediates"
Tsuruoka, Y. Shoda, K. Wada, T., Sekine, M.
J. Org. Chem. 65, 7479-7494 (2000).

"Synthesis and Properties of Aminoacylamido-AMP: Chemical Optimization for the Construction of an N-Acyl Phosphoramidate Linkage"
Moriguchi, T., Yanagi, T., Kunimori, M., Wada, T., Sekine, M.
J. Org. Chem. 65, 8229-8238 (2000).

"Synthesis and Antitumor activities of Phosmidosine A and its N-Acylated Derivative"
Moriguchi, T., Asai, N., Wada, T., Seio, K., Sasaki, T., Sekine M.
Tetrahedron Lett. 41, 5881-5885 (2000).

"Synthesis and Properties of 2'-O-Methyl-2-thiouridine and Oligoribonucleotides Containing 2'-O-Methyl-2-thiouridine"
Shoda, K., Okamoto, I., Wada, T., Seio, K., Sekine, M.
Bioorg. Med. Chem. Lett. 10, 1795-1798 (2000).

"Synthesis and Properties of Oligothymidylates Incorporating an Artificial Bend Motif"
Seio, K., Wada, T., Sekine, M.
Helv. Chim. Acta 83, 162-180 (2000).

"Solid-phase Synthesis of Deoxyribonucleotides Having a Cap Structure Analog"
Ushioda, M., Kadokura, M., Murami, Y. Moriguti, T., Wada, T., Seio, K., Sekine, M.
Nucleic Acids Symp. Ser. 44, 123-124 (2000).

"A Novel Approach to the Synthesis of Oligodeoxyribonucleotide Boranophosphates"
Wada, T., Shimizu, M., Oka, N. and Saigo, K.
Nucleic Acids Symp. Ser. 44, 135-136 (2000).

"Chemical Synthesis of Hydroxymethylphosphonateα-DNA"
Sato, Y., Tateno, G., Wada, T., Seio, K. and Sekine, M.
Nucleic Acids Symp. Ser. 44, 31-32 (2000).