東京理科大学 創域理工学部 生命生物科学科 中島研究室
脱水素酵素ファミリー（田口教授）
研究業績リスト
（*は責任著者、下線は研究室スタッフ、太字は研究室メンバー（筆頭著者と他一部のみ示しています。研究当時を含んでいます。））
＜主要な論文リスト＞
New glycoside hydrolase families of β-1,2-glucanases
Masahiro Nakajima*, Nobukiyo Tanaka, Sei Motouchi, Kaito Kobayashi, Hisaka Shimizu, Koichi Abe, Naoya Hosoyamada, Naoya Abara, Naoko Morimoto, Narumi Hiramoto, Ryosuke Nakata, Akira Takashima, Marie Hosoki, Soichiro Suzuki, Kako Shikano, Takahiro Fujimaru, Shiho Imagawa, Yukiya Kawadai, Ziyu Wang, Yoshinao Kitano, Takanori Nihira, Hiroyuki Nakai, Hayao Taguchi
Protein Science, 34 (6) e70147, 2025 (link)
Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium
Yutaka Nakazawa, Masumi Kageyama, Tomohiko Matsuzawa, Ziqin Liang, Kaito Kobayashi, Hisaka Shimizu, Kazuki Maeda, Miho Masuhiro, Sei Motouchi, Saika Kumano, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima*
Communications Biology, 8 Article number 66, 2025 (link)
Discovery of anomer-inverting transglycosylase: cyclic glucohexadecaose-producing enzyme from Xanthomonas, a phytopathogen
Sei Motouchi*, Shiro Komba, Hiroyuki Nakai, Masahiro Nakajima*
Journal of the American Chemical Society, 146 (26) 17738-17746, 2024 (link)
Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase
Nobukiyo Tanaka*, Ryotaro Saito, Kaito Kobayashi, Hiroyuki Nakai, Shogo Kamo, Kouji Kuramochi, Hayao Taguchi, Masahiro Nakajima*, Tomoko Masaike*
Applied Microbiology and Biotechnology, 108 (1) Article number 187, 2024 (link)
Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family
Sei Motouchi, Kaito Kobayashi, Hiroyuki Nakai, Masahiro Nakajima*
Communications Biology, 6 Article number 961, 2023 (link)
Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages
Kaito Kobayashi, Hisaka Shimizu, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Masahiro Nakajima*, Hayao Taguchi
Journal of Biological Chemistry, 298 (3) 101606, 2022 (link)
Identification, characterization and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Nobukiyo Tanaka, Masahiro Nakajima*, Megumi Narukawa-Nara, Hiroki Matsunaga, Shinji Kamisuki, Hiroki Aramasa, Yuta Takahashi, Naohisa Sugimoto, Koichi Abe, Tohru Terada, Akimasa Miyanaga, Tetsuro Yamashita, Fumio Sugawara, Takashi Kamakura, Shiro Komba, Hiroyuki Nakai, Hayao Taguchi
Journal of Biological Chemistry, 294 (19) 7942-7965, 2019 (link)
Structural basis of sequential allosteric transitions in tetrameric D-lactate dehydrogenases from three Gram-negative bacteria
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi*
Biochemistry, 57 (37) 5388-5406, 2018 (link)
Structural and thermodynamic insights into β-1,2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua
Koichi Abe, Naoki Sunagawa, Tohru Terada, Yuta Takahashi, Takatoshi Arakawa, Kiyohiko Igarashi, Masahiro Samejima, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima, Shinya Fushinobu*
Journal of Biological Chemistry, 293 (23) 8812-8828, 2018 (link)
Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Koichi Abe, Masahiro Nakajima*, Tetsuro Yamashita, Hiroki Matsunaga, Shinji Kamisuki, Takanori Nihira, Yuta Takahashi, Naohisa Sugimoto, Akimasa Miyanaga, Hiroyuki Nakai, Takatoshi Arakawa, Shinya Fushinobu, Hayao Taguchi
Journal of Biological Chemistry, 292 (18) 7487-7506, 2017 (link)
Large-scale preparation of 1,2-β-glucan using 1,2-β-oligoglucan phosphorylase
Koichi Abe, Masahiro Nakajima (co-first)*, Motomitsu Kitaoka, Hiroyuki Toyoizumi, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, and Hayao Taguchi
Journal of Applied Glycoscience, 62 (2) 47-52, 2015 (link)
The core of allosteric motion in Thermus caldophilus L-lactate dehydrogenase
Yoko Ikehara, Kazuhito Arai, Nayuta Furukawa, Tadashi Ohno, Tatsuya Miyake, Shinya Fushinobu, Masahiro Nakajima, Akimasa Miyanaga and Hayao Taguchi*
Journal of Biological Chemistry, 289 (45) 31550-31564, 2014 (link)
1,2-β-Oligoglucan phosphorylase from Listeria innocua
Masahiro Nakajima, Hiroyuki Toyoizumi (co-first), Koichi Abe, Hiroyuki Nakai, Hayao Taguchi, Motomitsu Kitaoka*
PLOS ONE, 9 (3) e92353, 2014 (link)
＜当研究室が主導した全論文リスト＞
open
New glycoside hydrolase families of β-1,2-glucanases
Masahiro Nakajima*, Nobukiyo Tanaka, Sei Motouchi, Kaito Kobayashi, Hisaka Shimizu, Koichi Abe, Naoya Hosoyamada, Naoya Abara, Naoko Morimoto, Narumi Hiramoto, Ryosuke Nakata, Akira Takashima, Marie Hosoki, Soichiro Suzuki, Kako Shikano, Takahiro Fujimaru, Shiho Imagawa, Yukiya Kawadai, Ziyu Wang, Yoshinao Kitano, Takanori Nihira, Hiroyuki Nakai, Hayao Taguchi
Protein Science, 34 (6) e70147, 2025
Enzymes that catalyze cyclization of β-1,2-glucans
Masahiro Nakajima*, Sei Motouchi, Nobukiyo Tanaka, Tomoko Masaike*
Applied Microbiology and Biotechnology, 109 Article number 49, 2025
Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium
Yutaka Nakazawa, Masumi Kageyama, Tomohiko Matsuzawa, Ziqin Liang, Kaito Kobayashi, Hisaka Shimizu, Kazuki Maeda, Miho Masuhiro, Sei Motouchi, Saika Kumano, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima*
Communications Biology, 8 Article number 66, 2025
Discovery of anomer-inverting transglycosylase: cyclic glucohexadecaose-producing enzyme from Xanthomonas, a phytopathogen
Sei Motouchi*, Shiro Komba, Hiroyuki Nakai, Masahiro Nakajima*
Journal of the American Chemical Society, 146 (26) 17738-17746, 2024
Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase
Nobukiyo Tanaka*, Ryotaro Saito, Kaito Kobayashi, Hiroyuki Nakai, Shogo Kamo, Kouji Kuramochi, Hayao Taguchi, Masahiro Nakajima*, Tomoko Masaike*
Applied Microbiology and Biotechnology, 108 (1) Article number 187, 2024
Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family
Sei Motouchi, Kaito Kobayashi, Hiroyuki Nakai,Masahiro Nakajima*
Communications Biology, 6 Article number 961, 2023
β-1,2-Glucans and associated enzymes
Masahiro Nakajima*
Biologia 78(7) 1741-1757, 2022 (read only version)
Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages
Kaito Kobayashi, Hisaka Shimizu, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Masahiro Nakajima*, Hayao Taguchi
Journal of Biological Chemistry, 298 (3) 101606, 2022
Enzymatic control and evaluation of degrees of polymerization of β-(1→2)-glucans
Masahiro Nakajima*, Nobukiyo Tanaka, Kaito Kobayashi, Hiroyuki Nakai, Satoshi Kimura, Tadashi Iwata, Hayao Taguchi
Analytical Biochemistry, 632 114366, 2021
Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose
Kaito Kobayashi, Masahiro Nakajima*, Hiroki Aramasa, Satoshi Kimura, Tadahisa Iwata, Hiroyuki Nakai, Hayao Taguchi
Bioscience, Biotechnology and Biochemistry, 83 (10) 1867-1874, 2019
Identification, characterization and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Nobukiyo Tanaka, Masahiro Nakajima*, Megumi Narukawa-Nara, Hiroki Matsunaga, Shinji Kamisuki, Hiroki Aramasa, Yuta Takahashi, Naohisa Sugimoto, Koichi Abe, Tohru Terada, Akimasa Miyanaga, Tetsuro Yamashita, Fumio Sugawara, Takashi Kamakura, Shiro Komba, Hiroyuki Nakai, Hayao Taguchi
Journal of Biological Chemistry, 294 (19) 7942-7965, 2019
Colorimetric determination of β-1,2-glucooligosaccharides for an enzymatic assay using 3-methyl-2-benzothiazolinonehydrazone
Kaito Kobayashi, Hiroki Aramasa, Hiroyuki Nakai, Masahiro Nakajima*, Hayao Taguchi
Analytical Biochemistry, 560 1-6, 2018
Synthesis of three deoxy-sophorose derivatives for evaluating the requirement of hydroxy groups at position 3 and/or 3' of sophorose by 1,2-β-oligoglucan phosphorylases
Nobukiyo Tanaka, Masahiro Nakajima, Hiroki Aramasa, Hiroyuki Nakai, Hayao Taguchi, Wakako Tsuzuki, Shiro Komba*
Carbohydrate Research, 468 13-22, 2018
Structural basis of sequential allosteric transitions in tetrameric D-lactate dehydrogenases from three Gram-negative bacteria
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi*
Biochemistry, 57 (37) 5388-5406, 2018
Characterization and structural analysis of a novel exo-type enzyme acting on β-1,2-glucooligosaccharides from Parabacteroides distasonis
Hisaka Shimizu, Masahiro Nakajima*, Akimasa Miyanaga, Yuta Takahashi, Nobukiyo Tanaka, Kaito Kobayashi, Naohisa Sugimoto, Hiroyuki Nakai, and Hayao Taguchi
Biochemistry, 57 (26) 3849-3860, 2018
Structural and thermodynamic insights into β-1,2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua
Koichi Abe, Naoki Sunagawa, Tohru Terada, Yuta Takahashi, Takatoshi Arakawa, Kiyohiko Igarashi, Masahiro Samejima, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima, Shinya Fushinobu*
Journal of Biological Chemistry, 293 (23) 8812-8828, 2018
Function and structure relationships of a β-1,2-glucooligosaccharide-degrading β-glucosidase
Rikuto Ishiguro, Nobukiyo Tanaka, Koichi Abe, Masahiro Nakajima*, Takuma Maeda, Akimasa Miyanaga, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, Hayao Taguchi
FEBS letters, 591 (23) 3926-3936, 2017
Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Koichi Abe, Masahiro Nakajima*, Tetsuro Yamashita, Hiroki Matsunaga, Shinji Kamisuki, Takanori Nihira, Yuta Takahashi, Naohisa Sugimoto, Akimasa Miyanaga, Hiroyuki Nakai, Takatoshi Arakawa, Shinya Fushinobu, Hayao Taguchi
Journal of Biological Chemistry, 292 (18) 7487-7506, 2017
The ternary complex structure of D-mandelate dehydrogenase with NADH and anilino(oxo)acetate
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi*
Biochemical and Biophysical Research Communications, 486 (3) 665-670, 2017
Mechanistic insight into the substrate specificity of 1,2-β-oligoglucan phosphorylase from Lachnoclostridium phytofermentans
Masahiro Nakajima*, Nobukiyo Tanaka, Nayuta Furukawa, Takanori Nihira, Yuki Kodutsumi, Yuta Takahashi, Naohisa Sugimoto, Akimasa Miyanaga, Shinya Fushinobu, Hayao Taguchi, Hiroyuki Nakai
Scientific reports, 7, Article number: 42671, 2017
Functional and structural analysis of a β-glucosidase involved in β-1,2-glucan metabolism in Listeria innocua
Masahiro Nakajima*, Ryuta Yoshida, Akimasa Miyanaga, Koichi Abe, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Toyoizumi, Hiroyuki Nakai, Motomitsu Kitaoka, Hayao Taguchi
PLOS ONE, 11 (2) e0148870, 2016
Large-scale preparation of 1,2-β-glucan using 1,2-β-oligoglucan phosphorylase
Koichi Abe, Masahiro Nakajima (co-first)*, Motomitsu Kitaoka, Hiroyuki Toyoizumi, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, and Hayao Taguchi
Journal of Applied Glycoscience, 62 (2) 47-52, 2015
The core of allosteric motion in Thermus caldophilus L-lactate dehydrogenase
Yoko Ikehara, Kazuhito Arai, Nayuta Furukawa, Tadashi Ohno, Tatsuya Miyake, Shinya Fushinobu, Masahiro Nakajima, Akimasa Miyanaga and Hayao Taguchi*
Journal of Biological Chemistry, 289 (45) 31550-31564, 2014
Diverse allosteric and catalytic functions of tetrameric D-lactate dehydrogenases from three Gram-negative bacteria
Nayuta Furukawa, Akimasa Miyanaga, Misato Togawa, Masahiro Nakajima, Hayao Taguchi*
AMB express, 4 (1) 76-87, 2014
Crystallization and preliminary X-ray diffraction analysis of Lin1840 protein, a putative β-glucosidase from Listeria innocua
Masahiro Nakajima*, Ryuta Yoshida, Akimasa Miyanaga, Hayao Taguchi
Acta Crystallographica Section F Structural Biology and Crystal Communication, 70 (10) 1398-1401, 2014
1,2-β-Oligoglucan phosphorylase from Listeria innocua
Masahiro Nakajima, Hiroyuki Toyoizumi (co-first), Koichi Abe, Hiroyuki Nakai, Hayao Taguchi, Motomitsu Kitaoka*
PLOS ONE, 9 (3) e92353, 2014
The crystal structure of D-mandelate dehydrogenase reveals its distinct substrate and coenzyme recognition mechanisms from those of 2-ketopantoate reductase
Akimasa Miyanaga, Shinsuke Fujisawa, Nayuta Furukawa, Kazuhito Arai, Masahiro Nakajima, Hayao Taguchi*
Biochemical and Biophysical Research Communications, 439 (1) 109-114, 2013
＜主に共同研究による論文＞
open
Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Meng-Shu Hao, Lauren S. McKee et al.
Nature Communications, 15 Article number 3429, 2024
Gasdermin D–mediated release of IL-33 from senescent hepatic stellate cells promotes obesity-associated hepatocellular carcinoma
Ryota Yamagishi, Eiji Hara, Naoko Ohtani, et al.
Science Immunology, 7 (72) eabl7209, 2022
Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation 2 in the rice blast fungus
Akihito Nozaka, Takashi Kamakura et al.
Scientific reports, 9, Article number: 9283, 2019
Chitin-deacetylase activity induces appressorium differentiation in the rice blast fungus Magnaporthe oryzae
Misa Kuroki, Takashi Kamakura et al.
Scientific reports, 7, Article number: 9697, 2017
Galacto-N-biose is neuroprotective against glutamate-induced excitotoxicity in vivo
Yo Shinoda*, Yui Nakajima, Hirotoshi Iguchi, Satoshi Tatsumi, Teiichi Furuichi, et al.
European Journal of Pharmacology, 791 711-717, 2016
Meng-Shu Hao, Lauren S. McKee et al.
Nature Communications, 15 Article number 3429, 2024
Gasdermin D–mediated release of IL-33 from senescent hepatic stellate cells promotes obesity-associated hepatocellular carcinoma
Ryota Yamagishi, Eiji Hara, Naoko Ohtani, et al.
Science Immunology, 7 (72) eabl7209, 2022
Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation 2 in the rice blast fungus
Akihito Nozaka, Takashi Kamakura et al.
Scientific reports, 9, Article number: 9283, 2019
Chitin-deacetylase activity induces appressorium differentiation in the rice blast fungus Magnaporthe oryzae
Misa Kuroki, Takashi Kamakura et al.
Scientific reports, 7, Article number: 9697, 2017
Galacto-N-biose is neuroprotective against glutamate-induced excitotoxicity in vivo
Yo Shinoda*, Yui Nakajima, Hirotoshi Iguchi, Satoshi Tatsumi, Teiichi Furuichi, et al.
European Journal of Pharmacology, 791 711-717, 2016
＜以下は研究室に赴任まで＞
open
A novel glycosylphosphatidylinositol-anchored glycoside hydrolase from Ustilago esculenta functions in β-1,3-glucan degradation
Masahiro Nakajima, Tetsuro Yamashita, Machiko Takahashi, Yuki Nakano, Takumi Takeda*
Applied and Environmental Microbiology, 78 (16) 5682-5689, 2012
Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes
Naotake Konno, Hideyuki Takahashi, Masahiro Nakajima, Takumi Takeda, Yuichi Sakamoto*
AMB Express, 2 (1) 29-35, 2012
Identification, cloning and characterization of β-glucosidase from Ustilago esculenta
Masahiro Nakajima, Tetsuro Yamashita, Machiko Takahashi, Yuki Nakano, Takumi Takeda*
Applied Microbiology and Biotechnology, 93 (5) 1989-1998, 2012
Enhanced saccharification by Trichoderma reesei expressing a β-glucosidase from Magnaporthe oryzae
Machiko Takahashi, Masahiro Nakajima, Yuki Nakano, Takumi Takeda*
Journal of Applied Glycoscience, 59 (2) 89-95, 2012
Bifidobacterium longum subsp. infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides
Erina Yoshida, Haruko Sakurama, Masashi Kiyohara, Masahiro Nakajima, Motomitsu Kitaoka, Hisashi Ashida, Junko Hirose, Takane Katayama*, Kenji Yamamoto, Hidehiko Kumagai
Glycobiology, 22 (3) 361-368, 2012
Identification of amino acid residues determining substrate preference of 1,3-β-galactosyl-N-acetylhexosamine phosphorylase
Mamoru Nishimoto, Masafumi Hidaka, Masahiro Nakajima, Shinya Fushinobu, Motomitsu Kitaoka*
Journal of Molecular Catalysis B: Enzymatic, 74 (1-2) 97-102, 2012
Practical preparation of D-galactosyl-β1,4-L-rhamnose employing combined action of phosphorylases
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Bioscience, Biotechnology and Biochemistry, 74 (8) 1652-1655, 2010
Characterization of D-galactosyl-β1→4-L-rhamnose phosphorylase from Opitutus terrae
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Enzyme and Microbial Technology, 46 (3-4), 315-319, 2010
Characterization of three β-galactoside phosphorylases from Clostridium phytofermentans: discovery of D-galactosyl-β1→4-L-rhamnose phosphorylase
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Journal of Biological Chemistry, 284 (29), 19220-19227, 2009
Characterization of β-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Applied Microbiology and Biotechnology, 83 (1), 109-115, 2009
Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6
Masahiro Nakajima, Motomitsu Kitaoka*
Applied and Environmental Microbiology, 74 (20) 6333-6337, 2008
Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124
Masahiro Nakajima, Takanori Nihira, Mamoru Nishimoto, Motomitsu Kitaoka*
Applied Microbiology and Biotechnology, 78 (3), 465-471, 2008
Colorimetric quantification of α-D-galactose 1-phosphate
Takanori Nihira, Masahiro Nakajima, Kousuke Inoue, Mamoru Nishimoto, Motomitsu Kitaoka*
Analytical Biochemistry, 371 (2), 259-261, 2007
Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima
Masahiro Nakajima, Shinya Fushinobu*, Hiromi Imamura, Hirofumi Shoun, Takayoshi Wakagi
Acta Crystallographica Section F Structural Biology and Crystal Communication, 62 (2), 104-105, 2006
Transglycosylation activity of Dictyoglomus thermophilum amylase A
Masahiro Nakajima, Hiromi Imamura, Sueharu Horinouchi, Hirofumi Shoun, Takayoshi Wakagi*
Bioscience, Biotechnology and Biochemistry, 68 (11), 2369-2373, 2004
Unique metal dependency of cytosolic α-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium
Masahiro Nakajima, Hiromi Imamura, Hirofumi Shoun, Takayoshi Wakagi*
Archives of Biochemistry and Biophysics, 415 (1), 87-93, 2003
Masahiro Nakajima, Tetsuro Yamashita, Machiko Takahashi, Yuki Nakano, Takumi Takeda*
Applied and Environmental Microbiology, 78 (16) 5682-5689, 2012
Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes
Naotake Konno, Hideyuki Takahashi, Masahiro Nakajima, Takumi Takeda, Yuichi Sakamoto*
AMB Express, 2 (1) 29-35, 2012
Identification, cloning and characterization of β-glucosidase from Ustilago esculenta
Masahiro Nakajima, Tetsuro Yamashita, Machiko Takahashi, Yuki Nakano, Takumi Takeda*
Applied Microbiology and Biotechnology, 93 (5) 1989-1998, 2012
Enhanced saccharification by Trichoderma reesei expressing a β-glucosidase from Magnaporthe oryzae
Machiko Takahashi, Masahiro Nakajima, Yuki Nakano, Takumi Takeda*
Journal of Applied Glycoscience, 59 (2) 89-95, 2012
Bifidobacterium longum subsp. infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides
Erina Yoshida, Haruko Sakurama, Masashi Kiyohara, Masahiro Nakajima, Motomitsu Kitaoka, Hisashi Ashida, Junko Hirose, Takane Katayama*, Kenji Yamamoto, Hidehiko Kumagai
Glycobiology, 22 (3) 361-368, 2012
Identification of amino acid residues determining substrate preference of 1,3-β-galactosyl-N-acetylhexosamine phosphorylase
Mamoru Nishimoto, Masafumi Hidaka, Masahiro Nakajima, Shinya Fushinobu, Motomitsu Kitaoka*
Journal of Molecular Catalysis B: Enzymatic, 74 (1-2) 97-102, 2012
Practical preparation of D-galactosyl-β1,4-L-rhamnose employing combined action of phosphorylases
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Bioscience, Biotechnology and Biochemistry, 74 (8) 1652-1655, 2010
Characterization of D-galactosyl-β1→4-L-rhamnose phosphorylase from Opitutus terrae
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Enzyme and Microbial Technology, 46 (3-4), 315-319, 2010
Characterization of three β-galactoside phosphorylases from Clostridium phytofermentans: discovery of D-galactosyl-β1→4-L-rhamnose phosphorylase
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Journal of Biological Chemistry, 284 (29), 19220-19227, 2009
Characterization of β-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Applied Microbiology and Biotechnology, 83 (1), 109-115, 2009
Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6
Masahiro Nakajima, Motomitsu Kitaoka*
Applied and Environmental Microbiology, 74 (20) 6333-6337, 2008
Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124
Masahiro Nakajima, Takanori Nihira, Mamoru Nishimoto, Motomitsu Kitaoka*
Applied Microbiology and Biotechnology, 78 (3), 465-471, 2008
Colorimetric quantification of α-D-galactose 1-phosphate
Takanori Nihira, Masahiro Nakajima, Kousuke Inoue, Mamoru Nishimoto, Motomitsu Kitaoka*
Analytical Biochemistry, 371 (2), 259-261, 2007
Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima
Masahiro Nakajima, Shinya Fushinobu*, Hiromi Imamura, Hirofumi Shoun, Takayoshi Wakagi
Acta Crystallographica Section F Structural Biology and Crystal Communication, 62 (2), 104-105, 2006
Transglycosylation activity of Dictyoglomus thermophilum amylase A
Masahiro Nakajima, Hiromi Imamura, Sueharu Horinouchi, Hirofumi Shoun, Takayoshi Wakagi*
Bioscience, Biotechnology and Biochemistry, 68 (11), 2369-2373, 2004
Unique metal dependency of cytosolic α-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium
Masahiro Nakajima, Hiromi Imamura, Hirofumi Shoun, Takayoshi Wakagi*
Archives of Biochemistry and Biophysics, 415 (1), 87-93, 2003
＜受賞＞
2016年度 田中信清 日本応用糖質科学会平成28年度大会 ポスター賞2016年度 阿部紘一 日本応用糖質科学会平成28年度大会 ポスター賞
2016年度 阿部紘一 平成28年度日本応用糖質科学会東日本支部若手奨励賞
2017年度 阿部紘一 東京大学大学院農学生命科学研究科 研究科長賞
2018年度 小林海渡 日本農芸化学会関東支部2018年度大会 優秀発表賞（口頭発表部門）
2019年度 中島将博 第21回 酵素応用シンポジウム 研究奨励賞
2020年度 中島将博 日本応用糖質科学会2020年度（第69回）大会 奨励賞
2022年度 元内省 日本応用糖質科学会2022年度（第71回）大会 ポスター賞
2023年度 元内省 日本農芸化学会2024年度大会 トピックス賞
2024年度 田中信清 日本応用糖質科学会 東日本支部 若手奨励賞
2024年度 元内省 PF-AU学生論文賞
2024年度 元内省 創域理工学部長・創域理工学研究科長表彰
2025年度 元内省 日本応用糖質科学会東日本支部若手奨励賞
＜その他＞
・研究室で発見した糖質関連酵素の新規グループ---GH144（解説ページ、阿部博士, 2014年度（修士）修了）
---GH162（解説ページ、田中博士, 2018年度修了）
---GH186（解説ページ、元内博士, 2024年度修了）
---GH189（解説ページ、田中博士, GH162と同じ）
---GH192, GH193, GH194（解説ページ（予定）、中島（予定））
・プレスリリースなど
（１）新規な糖質加水分解酵素「エンド-β-1,2-グルカナーゼ」の同定と機能構造解析
（２）新規な分子進化のルーツを持つ糖鎖分解酵素を発見
（３）希少なオリゴ糖“β-1,2-グルコオリゴ糖”を細菌が捕まえるしくみを解明
（４）グルコースが連なったオリゴ糖に作用する新規な糖転移酵素の発見 ～新規なオリゴ糖配糖体の合成法の開発に貢献～
（５）細菌の病原性などに関わる糖鎖「OPG」の新規の生合成酵素を発見 ～新たな創薬ターゲットとなる可能性～
-----(Novel Enzyme Family Could Provide Insights into Bacterial Pathogenicity)
（６）β-1,2-グルカンの環化に関わるメカニズムを解明 ～生理学的に重要な環状β-1,2-グルカン研究の進展に寄与～
-----(Uncovering The Cyclization Mechanism of Cyclic β-1,2-Glucan Synthase)
（７）糖質加水分解酵素群の100年を超える研究の歴史の中で初めて見つかった糖転移反応
-----～植物に対する病原性因子の環状糖を合成するアノマー反転型糖転移反応の発見～
-----(Breakthrough in Plant Disease: New Enzyme Could Lead to Anti-Bacterial Pesticides)
（８）希少なオリゴ糖に作用するユニークな基質特性を持つ新規酵素を発見
-----～新たな機能を持つ糖鎖の合成、利用の可能性を拓く～
-----(Novel Enzyme Found in Gut Bacteria Could Revolutionize Prebiotic Research)
（９）系統的に新規なβ-1,2-グルカナーゼ群の発見
----- ～酵素の構造解析・機能解析から解明された分子進化の手がかり～
-----(Scientists Discover New Enzyme Families That Break Down Rare Bacterial Carbohydrates)
・酵素の新規反応の発見（EC番号）
---EC 2.4.1.247
---EC 2.4.1.333
---EC 3.2.1.214
---EC 2.4.1.391
---EC 2.4.1.397
---EC 2.4.1.-- 申請準備中
---EC 3.2.1.-- 申請準備中
・総説、紹介記事
---糖質面白話への寄稿（日本応用糖質科学会）
---細菌が広く共生，病原性のために合成する糖鎖の合成酵素ファミリーに関する構造生物学的洞察（日本結晶学会 最近の研究動向）