（*は責任著者、下線は研究室スタッフ、太字は研究室メンバー（筆頭著者と他一部のみ示しています。研究当時を含んでいます。））

New glycoside hydrolase families of β-1,2-glucanases
Masahiro Nakajima*, Nobukiyo Tanaka, Sei Motouchi, Kaito Kobayashi, Hisaka Shimizu, Koichi Abe, Naoya Hosoyamada, Naoya Abara, Naoko Morimoto, Narumi Hiramoto, Ryosuke Nakata, Akira Takashima, Marie Hosoki, Soichiro Suzuki, Kako Shikano, Takahiro Fujimaru, Shiho Imagawa, Yukiya Kawadai, Ziyu Wang, Yoshinao Kitano, Takanori Nihira, Hiroyuki Nakai, Hayao Taguchi
Protein Science, 34 (6) e70147, 2025 (link)

Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium
Yutaka Nakazawa, Masumi Kageyama, Tomohiko Matsuzawa, Ziqin Liang, Kaito Kobayashi, Hisaka Shimizu, Kazuki Maeda, Miho Masuhiro, Sei Motouchi, Saika Kumano, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima*
Communications Biology, 8 (1) Article number 66, 2025 (link)

Discovery of anomer-inverting transglycosylase: cyclic glucohexadecaose-producing enzyme from Xanthomonas, a phytopathogen
Sei Motouchi*, Shiro Komba, Hiroyuki Nakai, Masahiro Nakajima*
Journal of the American Chemical Society, 146 (26) 17738-17746, 2024 (link)

Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase
Nobukiyo Tanaka*, Ryotaro Saito, Kaito Kobayashi, Hiroyuki Nakai, Shogo Kamo, Kouji Kuramochi, Hayao Taguchi, Masahiro Nakajima*, Tomoko Masaike*
Applied Microbiology and Biotechnology, 108 (1) Article number 187, 2024 (link)

Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family
Sei Motouchi, Kaito Kobayashi, Hiroyuki Nakai, Masahiro Nakajima*
Communications Biology, 6 Article number 961, 2023 (link)

Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages
Kaito Kobayashi, Hisaka Shimizu, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Masahiro Nakajima*, Hayao Taguchi
Journal of Biological Chemistry, 298 (3) 101606, 2022 (link)

Identification, characterization and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Nobukiyo Tanaka, Masahiro Nakajima*, Megumi Narukawa-Nara, Hiroki Matsunaga, Shinji Kamisuki, Hiroki Aramasa, Yuta Takahashi, Naohisa Sugimoto, Koichi Abe, Tohru Terada, Akimasa Miyanaga, Tetsuro Yamashita, Fumio Sugawara, Takashi Kamakura, Shiro Komba, Hiroyuki Nakai, Hayao Taguchi
Journal of Biological Chemistry, 294 (19) 7942-7965, 2019 (link)

Structural basis of sequential allosteric transitions in tetrameric D-lactate dehydrogenases from three Gram-negative bacteria
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi*
Biochemistry, 57 (37) 5388-5406, 2018 (link)

Structural and thermodynamic insights into β-1,2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua
Koichi Abe, Naoki Sunagawa, Tohru Terada, Yuta Takahashi, Takatoshi Arakawa, Kiyohiko Igarashi, Masahiro Samejima, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima, Shinya Fushinobu*
Journal of Biological Chemistry, 293 (23) 8812-8828, 2018 (link)

Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Koichi Abe, Masahiro Nakajima*, Tetsuro Yamashita, Hiroki Matsunaga, Shinji Kamisuki, Takanori Nihira, Yuta Takahashi, Naohisa Sugimoto, Akimasa Miyanaga, Hiroyuki Nakai, Takatoshi Arakawa, Shinya Fushinobu, Hayao Taguchi
Journal of Biological Chemistry, 292 (18) 7487-7506, 2017 (link)

Large-scale preparation of 1,2-β-glucan using 1,2-β-oligoglucan phosphorylase
Koichi Abe, Masahiro Nakajima (co-first)*, Motomitsu Kitaoka, Hiroyuki Toyoizumi, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, and Hayao Taguchi
Journal of Applied Glycoscience, 62 (2) 47-52, 2015 (link)

The core of allosteric motion in Thermus caldophilus L-lactate dehydrogenase
Yoko Ikehara, Kazuhito Arai, Nayuta Furukawa, Tadashi Ohno, Tatsuya Miyake, Shinya Fushinobu, Masahiro Nakajima, Akimasa Miyanaga and Hayao Taguchi*
Journal of Biological Chemistry, 289 (45) 31550-31564, 2014 (link)

1,2-β-Oligoglucan phosphorylase from Listeria innocua
Masahiro Nakajima, Hiroyuki Toyoizumi (co-first), Koichi Abe, Hiroyuki Nakai, Hayao Taguchi, Motomitsu Kitaoka*
PLOS ONE, 9 (3) e92353, 2014 (link)

A β‐1,2‐glucan‐associated glycoside hydrolase family 1 β‐glucosidase from Streptomyces griseus
Haruto Kumakura, Sei Motouchi, Kaito Kobayashi, Miyu Inoue, Natsuki Kariuda, Hiroyuki Nakai, Masahiro Nakajima*
Protein Science, 34 (9) e70255, 2025

New glycoside hydrolase families of β-1,2-glucanases
Masahiro Nakajima*, Nobukiyo Tanaka, Sei Motouchi, Kaito Kobayashi, Hisaka Shimizu, Koichi Abe, Naoya Hosoyamada, Naoya Abara, Naoko Morimoto, Narumi Hiramoto, Ryosuke Nakata, Akira Takashima, Marie Hosoki, Soichiro Suzuki, Kako Shikano, Takahiro Fujimaru, Shiho Imagawa, Yukiya Kawadai, Ziyu Wang, Yoshinao Kitano, Takanori Nihira, Hiroyuki Nakai, Hayao Taguchi
Protein Science, 34 (6) e70147, 2025

Enzymes that catalyze cyclization of β-1,2-glucans
Masahiro Nakajima*, Sei Motouchi, Nobukiyo Tanaka, Tomoko Masaike*
Applied Microbiology and Biotechnology, 109 Article number 49, 2025

Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium
Yutaka Nakazawa, Masumi Kageyama, Tomohiko Matsuzawa, Ziqin Liang, Kaito Kobayashi, Hisaka Shimizu, Kazuki Maeda, Miho Masuhiro, Sei Motouchi, Saika Kumano, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima*
Communications Biology, 8 Article number 66, 2025

Discovery of anomer-inverting transglycosylase: cyclic glucohexadecaose-producing enzyme from Xanthomonas, a phytopathogen
Sei Motouchi*, Shiro Komba, Hiroyuki Nakai, Masahiro Nakajima*
Journal of the American Chemical Society, 146 (26) 17738-17746, 2024

Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase
Nobukiyo Tanaka*, Ryotaro Saito, Kaito Kobayashi, Hiroyuki Nakai, Shogo Kamo, Kouji Kuramochi, Hayao Taguchi, Masahiro Nakajima*, Tomoko Masaike*
Applied Microbiology and Biotechnology, 108 (1) Article number 187, 2024

Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family
Sei Motouchi, Kaito Kobayashi, Hiroyuki Nakai,Masahiro Nakajima*
Communications Biology, 6 Article number 961, 2023

β-1,2-Glucans and associated enzymes
Masahiro Nakajima*
Biologia 78(7) 1741-1757, 2023

Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages
Kaito Kobayashi, Hisaka Shimizu, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Masahiro Nakajima*, Hayao Taguchi
Journal of Biological Chemistry, 298 (3) 101606, 2022

Enzymatic control and evaluation of degrees of polymerization of β-(1→2)-glucans
Masahiro Nakajima*, Nobukiyo Tanaka, Kaito Kobayashi, Hiroyuki Nakai, Satoshi Kimura, Tadashi Iwata, Hayao Taguchi
Analytical Biochemistry, 632 114366, 2021

Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose
Kaito Kobayashi, Masahiro Nakajima*, Hiroki Aramasa, Satoshi Kimura, Tadahisa Iwata, Hiroyuki Nakai, Hayao Taguchi
Bioscience, Biotechnology and Biochemistry, 83 (10) 1867-1874, 2019

Identification, characterization and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Nobukiyo Tanaka, Masahiro Nakajima*, Megumi Narukawa-Nara, Hiroki Matsunaga, Shinji Kamisuki, Hiroki Aramasa, Yuta Takahashi, Naohisa Sugimoto, Koichi Abe, Tohru Terada, Akimasa Miyanaga, Tetsuro Yamashita, Fumio Sugawara, Takashi Kamakura, Shiro Komba, Hiroyuki Nakai, Hayao Taguchi
Journal of Biological Chemistry, 294 (19) 7942-7965, 2019

Colorimetric determination of β-1,2-glucooligosaccharides for an enzymatic assay using 3-methyl-2-benzothiazolinonehydrazone
Kaito Kobayashi, Hiroki Aramasa, Hiroyuki Nakai, Masahiro Nakajima*, Hayao Taguchi
Analytical Biochemistry, 560 1-6, 2018

Synthesis of three deoxy-sophorose derivatives for evaluating the requirement of hydroxy groups at position 3 and/or 3' of sophorose by 1,2-β-oligoglucan phosphorylases
Nobukiyo Tanaka, Masahiro Nakajima, Hiroki Aramasa, Hiroyuki Nakai, Hayao Taguchi, Wakako Tsuzuki, Shiro Komba*
Carbohydrate Research, 468 13-22, 2018

Structural basis of sequential allosteric transitions in tetrameric D-lactate dehydrogenases from three Gram-negative bacteria
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi*
Biochemistry, 57 (37) 5388-5406, 2018

Characterization and structural analysis of a novel exo-type enzyme acting on β-1,2-glucooligosaccharides from Parabacteroides distasonis
Hisaka Shimizu, Masahiro Nakajima*, Akimasa Miyanaga, Yuta Takahashi, Nobukiyo Tanaka, Kaito Kobayashi, Naohisa Sugimoto, Hiroyuki Nakai, and Hayao Taguchi
Biochemistry, 57 (26) 3849-3860, 2018

Structural and thermodynamic insights into β-1,2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua
Koichi Abe, Naoki Sunagawa, Tohru Terada, Yuta Takahashi, Takatoshi Arakawa, Kiyohiko Igarashi, Masahiro Samejima, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima, Shinya Fushinobu*
Journal of Biological Chemistry, 293 (23) 8812-8828, 2018

Function and structure relationships of a β-1,2-glucooligosaccharide-degrading β-glucosidase
Rikuto Ishiguro, Nobukiyo Tanaka, Koichi Abe, Masahiro Nakajima*, Takuma Maeda, Akimasa Miyanaga, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, Hayao Taguchi
FEBS letters, 591 (23) 3926-3936, 2017

Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family
Koichi Abe, Masahiro Nakajima*, Tetsuro Yamashita, Hiroki Matsunaga, Shinji Kamisuki, Takanori Nihira, Yuta Takahashi, Naohisa Sugimoto, Akimasa Miyanaga, Hiroyuki Nakai, Takatoshi Arakawa, Shinya Fushinobu, Hayao Taguchi
Journal of Biological Chemistry, 292 (18) 7487-7506, 2017

The ternary complex structure of D-mandelate dehydrogenase with NADH and anilino(oxo)acetate
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi*
Biochemical and Biophysical Research Communications, 486 (3) 665-670, 2017

Mechanistic insight into the substrate specificity of 1,2-β-oligoglucan phosphorylase from Lachnoclostridium phytofermentans
Masahiro Nakajima*, Nobukiyo Tanaka, Nayuta Furukawa, Takanori Nihira, Yuki Kodutsumi, Yuta Takahashi, Naohisa Sugimoto, Akimasa Miyanaga, Shinya Fushinobu, Hayao Taguchi, Hiroyuki Nakai
Scientific Reports, 7, Article number: 42671, 2017

Functional and structural analysis of a β-glucosidase involved in β-1,2-glucan metabolism in Listeria innocua
Masahiro Nakajima*, Ryuta Yoshida, Akimasa Miyanaga, Koichi Abe, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Toyoizumi, Hiroyuki Nakai, Motomitsu Kitaoka, Hayao Taguchi
PLOS ONE, 11 (2) e0148870, 2016

Large-scale preparation of 1,2-β-glucan using 1,2-β-oligoglucan phosphorylase
Koichi Abe, Masahiro Nakajima (co-first)*, Motomitsu Kitaoka, Hiroyuki Toyoizumi, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, and Hayao Taguchi
Journal of Applied Glycoscience, 62 (2) 47-52, 2015

The core of allosteric motion in Thermus caldophilus L-lactate dehydrogenase
Yoko Ikehara, Kazuhito Arai, Nayuta Furukawa, Tadashi Ohno, Tatsuya Miyake, Shinya Fushinobu, Masahiro Nakajima, Akimasa Miyanaga and Hayao Taguchi*
Journal of Biological Chemistry, 289 (45) 31550-31564, 2014

Diverse allosteric and catalytic functions of tetrameric D-lactate dehydrogenases from three Gram-negative bacteria
Nayuta Furukawa, Akimasa Miyanaga, Misato Togawa, Masahiro Nakajima, Hayao Taguchi*
AMB express, 4 (1) 76-87, 2014

Crystallization and preliminary X-ray diffraction analysis of Lin1840 protein, a putative β-glucosidase from Listeria innocua
Masahiro Nakajima*, Ryuta Yoshida, Akimasa Miyanaga, Hayao Taguchi
Acta Crystallographica Section F Structural Biology and Crystal Communication, 70 (10) 1398-1401, 2014

1,2-β-Oligoglucan phosphorylase from Listeria innocua
Masahiro Nakajima, Hiroyuki Toyoizumi (co-first), Koichi Abe, Hiroyuki Nakai, Hayao Taguchi, Motomitsu Kitaoka*
PLOS ONE, 9 (3) e92353, 2014

The crystal structure of D-mandelate dehydrogenase reveals its distinct substrate and coenzyme recognition mechanisms from those of 2-ketopantoate reductase
Akimasa Miyanaga, Shinsuke Fujisawa, Nayuta Furukawa, Kazuhito Arai, Masahiro Nakajima, Hayao Taguchi*
Biochemical and Biophysical Research Communications, 439 (1) 109-114, 2013

Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Meng-Shu Hao, Lauren S. McKee et al.
Nature Communications, 15 Article number 3429, 2024

Gasdermin D–mediated release of IL-33 from senescent hepatic stellate cells promotes obesity-associated hepatocellular carcinoma
Ryota Yamagishi, Eiji Hara, Naoko Ohtani, et al.
Science Immunology, 7 (72) eabl7209, 2022

Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation 2 in the rice blast fungus
Akihito Nozaka, Takashi Kamakura et al.
Scientific reports, 9, Article number: 9283, 2019

Chitin-deacetylase activity induces appressorium differentiation in the rice blast fungus Magnaporthe oryzae
Misa Kuroki, Takashi Kamakura et al.
Scientific reports, 7, Article number: 9697, 2017

Galacto-N-biose is neuroprotective against glutamate-induced excitotoxicity in vivo
Yo Shinoda*, Yui Nakajima, Hirotoshi Iguchi, Satoshi Tatsumi, Teiichi Furuichi, et al.
European Journal of Pharmacology, 791 711-717, 2016

A novel glycosylphosphatidylinositol-anchored glycoside hydrolase from Ustilago esculenta functions in β-1,3-glucan degradation
Masahiro Nakajima, Tetsuro Yamashita, Machiko Takahashi, Yuki Nakano, Takumi Takeda*
Applied and Environmental Microbiology, 78 (16) 5682-5689, 2012

Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes
Naotake Konno, Hideyuki Takahashi, Masahiro Nakajima, Takumi Takeda, Yuichi Sakamoto*
AMB Express, 2 (1) 29-35, 2012

Identification, cloning and characterization of β-glucosidase from Ustilago esculenta
Masahiro Nakajima, Tetsuro Yamashita, Machiko Takahashi, Yuki Nakano, Takumi Takeda*
Applied Microbiology and Biotechnology, 93 (5) 1989-1998, 2012

Enhanced saccharification by Trichoderma reesei expressing a β-glucosidase from Magnaporthe oryzae
Machiko Takahashi, Masahiro Nakajima, Yuki Nakano, Takumi Takeda*
Journal of Applied Glycoscience, 59 (2) 89-95, 2012

Bifidobacterium longum subsp. infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides
Erina Yoshida, Haruko Sakurama, Masashi Kiyohara, Masahiro Nakajima, Motomitsu Kitaoka, Hisashi Ashida, Junko Hirose, Takane Katayama*, Kenji Yamamoto, Hidehiko Kumagai
Glycobiology, 22 (3) 361-368, 2012

Identification of amino acid residues determining substrate preference of 1,3-β-galactosyl-N-acetylhexosamine phosphorylase
Mamoru Nishimoto, Masafumi Hidaka, Masahiro Nakajima, Shinya Fushinobu, Motomitsu Kitaoka*
Journal of Molecular Catalysis B: Enzymatic, 74 (1-2) 97-102, 2012

Practical preparation of D-galactosyl-β1,4-L-rhamnose employing combined action of phosphorylases
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Bioscience, Biotechnology and Biochemistry, 74 (8) 1652-1655, 2010

Characterization of D-galactosyl-β1→4-L-rhamnose phosphorylase from Opitutus terrae
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Enzyme and Microbial Technology, 46 (3-4), 315-319, 2010

Characterization of three β-galactoside phosphorylases from Clostridium phytofermentans: discovery of D-galactosyl-β1→4-L-rhamnose phosphorylase
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Journal of Biological Chemistry, 284 (29), 19220-19227, 2009

Characterization of β-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes
Masahiro Nakajima, Mamoru Nishimoto, Motomitsu Kitaoka*
Applied Microbiology and Biotechnology, 83 (1), 109-115, 2009

Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6
Masahiro Nakajima, Motomitsu Kitaoka*
Applied and Environmental Microbiology, 74 (20) 6333-6337, 2008

Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124
Masahiro Nakajima, Takanori Nihira, Mamoru Nishimoto, Motomitsu Kitaoka*
Applied Microbiology and Biotechnology, 78 (3), 465-471, 2008

Colorimetric quantification of α-D-galactose 1-phosphate
Takanori Nihira, Masahiro Nakajima, Kousuke Inoue, Mamoru Nishimoto, Motomitsu Kitaoka*
Analytical Biochemistry, 371 (2), 259-261, 2007

Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima
Masahiro Nakajima, Shinya Fushinobu*, Hiromi Imamura, Hirofumi Shoun, Takayoshi Wakagi
Acta Crystallographica Section F Structural Biology and Crystal Communication, 62 (2), 104-105, 2006

Transglycosylation activity of Dictyoglomus thermophilum amylase A
Masahiro Nakajima, Hiromi Imamura, Sueharu Horinouchi, Hirofumi Shoun, Takayoshi Wakagi*
Bioscience, Biotechnology and Biochemistry, 68 (11), 2369-2373, 2004

Unique metal dependency of cytosolic α-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium
Masahiro Nakajima, Hiromi Imamura, Hirofumi Shoun, Takayoshi Wakagi*
Archives of Biochemistry and Biophysics, 415 (1), 87-93, 2003