- 2021年8月 Webinar Universe 10 in FIBER日本核酸化学会若手フォーラム FIBER核酸化学若手講演賞、日本核酸化学会・甲南大学先端生命工学研究所（FIBER）
- 2018年4月 日本化学会第98春季年会(2018) 優秀講演賞(学術)、日本化学会
- 2012年4月 日本化学会第92春季年会(2012) 学生講演賞、日本化学会
- 2011年1月 第5回リバネス研究費 オンチップ・バイオテクノロジーズ賞、リバネス
- 2010年1月 The 37th International Symposium on Nucleic Acids Chemistry 2010, Poster Award for Young Scientist、International Symposium on Nucleic Acids Chemistry
Achievement業績
2025年度
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Functional and Structural Analyses of Diverse G-Quadruplex and Non-G-Quadruplex Structures Formed by Guanine-Rich Nucleic Acids: A Study on the Insulin Aptamer
Iwano N, Oyama T, Tomizawa M, Inaba S, Mori R, Nakazawa Y, Sode K, Tsukakoshi K, Nakano T, Ikebukuro K.
Small. 2025 Jun 16:e2501336. doi: 10.1002/smll.202501336. Online ahead of print.
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Bright and pH-sensitive Baby Spinach aptamer with RNA triplex fusion
Ueno K, Tsukakoshi K, Takeuchi N, Inaba S, Idili A, Porchetta A, Ricci F, Ikebukuro K.
Nucleic Acids Res. 2025 Jun 6;53(11):gkaf151. doi: 10.1093/nar/gkaf151.
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Development of a mertansine-specific DNA aptamer and novel high-throughput sandwich enzyme-linked oligonucleotide assay for quantification and characterization of trastuzumab emtansine
Yamada T, Furusho A, Kojima K, Sugiyama E, Mizuno H, Tsukakoshi K, Hayashi H, Yamano T, Hasebe T, Toyo'oka T, Ikebukuro K, Todoroki K.
Biosens Bioelectron. 2025 Mar 15;272:117108. doi: 10.1016/j.bios.2024.117108. Epub 2024 Dec 28.
2024年度
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Proximity-Unlocked Luminescence by Sequential Enzymatic Reactions from Antibody and Antibody/Aptamer (PULSERAA): A Platform for Detection and Visualization of Virus-Containing Spots
Miura D, Hayashi W, Hirano K, Sasaki I, Tsukakoshi K, Kakizoe H, Asai S, Vavricka CJ, Takemae H, Mizutani T, Tsugawa W, Sode K, Ikebukuro K, Asano R.
Adv Sci (Weinh). 2024 Nov;11(43):e2403871. doi: 10.1002/advs.202403871. Epub 2024 Sep 24.
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Simple and fast one-step FRET assay of therapeutic mAb bevacizumab using anti-idiotype DNA aptamer for process analytical technology
Yamada T, Tsukakoshi K, Furusho A, Sugiyama E, Mizuno H, Hayashi H, Yamano T, Kumobayashi H, Hasebe T, Ikebukuro K, Toyo'oka T, Todoroki K.
Talanta. 2024 Sep 1;277:126349. doi: 10.1016/j.talanta.2024.126349. Epub 2024 Jun 4.
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Structure of cytotoxic amyloid oligomers generated during disaggregation
Kaku T, Ikebukuro K, Tsukakoshi K.
J Biochem. 2024 May 31;175(6):575-585. doi: 10.1093/jb/mvae023.
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Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA-Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element
Komiya E, Takamatsu S, Miura D, Tsukakoshi K, Tsugawa W, Sode K, Ikebukuro K, Asano R.
J Am Chem Soc. 2024 Feb 14;146(6):4087-4097. doi: 10.1021/jacs.3c12668. Epub 2024 Jan 31.
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Aptamer-enhanced particle aggregation inhibition assay for simple homogeneous protein detection using DNA aptamer and thermo-responsive magnetic nanoparticles
Rokutani S, Hiraka K, Saitoh H, Saito T, Nonaka Y, Ueno K, Tsukakoshi K, Ohnishi N, Ikebukuro K.
Biosens Bioelectron. 2024 Feb 1;245:115827. doi: 10.1016/j.bios.2023.115827. Epub 2023 Nov 10.
2023年度
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Real-time monitoring of the amyloid β(1-42) monomer-to-oligomer channel transition using a lipid bilayer system
Numaguchi Y, Tsukakoshi K, Takeuchi N, Suzuki Y, Ikebukuro K, Kawano R.
PNAS Nexus. 2023 Dec 14;3(1):pgad437. doi: 10.1093/pnasnexus/pgad437. eCollection 2024 Jan.
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Development of a liquid chromatography-based versatile bioanalysis for bevacizumab based on pretreatment combining aptamer affinity purification and centrifugal ultrafiltration concentration
Todoroki K, Hamada D, Yamada T, Saito T, Shimizu Y, Sugiyama E, Mizuno H, Hayashi H, Tsukakoshi K, Ikebukuro K.
Anal Sci. 2023 Nov;39(11):1805-1811. doi: 10.1007/s44211-023-00417-2. Epub 2023 Sep 3.
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Identification of novel amyloidosis in dogs: α-S1-casein acquires amyloidogenicity in mammary tumor by overexpression and N-terminal truncation
Murakami T, Kaku T, Tsukakoshi K, Iwaide S, Itoh Y, Hisada M, Nomura K, Kubo R, Ikebukuro K, Sassa-O'Brien Y, Kametani F.
Vet Pathol. 2023 Mar;60(2):203-213. doi: 10.1177/03009858221148511. Epub 2023 Jan 21.
2022年度
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Development of Alkaline Phosphatase-Fused Mouse Prion Protein and Its Application in Toxic Aβ Oligomer Detection
Tsukakoshi K, Kubo R, Ikebukuro K.
Int J Mol Sci. 2022 Nov 23;23(23):14588. doi: 10.3390/ijms232314588.
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Enhancement of DNAzymatic activity using iterative in silico maturation
Fenati RA, Chen Z, Yamagishi Y, Tsukakoshi K, Ikebukuor K, Manian A, Russo SP, Yamazaki T, Ellis AV.
J Mater Chem B. 2022 Nov 9;10(43):8960-8969. doi: 10.1039/d2tb01638a.
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Effects of G-Quadruplex Ligands on the Topology, Stability, and Immunostimulatory Properties of G-Quadruplex-Based CpG Oligodeoxynucleotides
Tu ATT, Hoshi K, Ma Y, Oyama T, Suzuki S, Tsukakoshi K, Nagasawa K, Ikebukuro K, Yamazaki T.
ACS Chem Biol. 2022 Jul 15;17(7):1703-1713. doi: 10.1021/acschembio.1c00904. Epub 2022 Jun 29.
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CpG Methylation Altered the Stability and Structure of the i-Motifs Located in the CpG Islands
Oshikawa D, Inaba S, Kitagawa Y, Tsukakoshi K, Ikebukuro K.
Int J Mol Sci. 2022 Jun 9;23(12):6467. doi: 10.3390/ijms23126467.
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Development of a DNA aptamer that binds to the complementarity-determining region of therapeutic monoclonal antibody and affinity improvement induced by pH-change for sensitive detection
Saito T, Shimizu Y, Tsukakoshi K, Abe K, Lee J, Ueno K, Asano R, Jones BV, Yamada T, Nakano T, Tong J, Hishiki A, Hara K, Hashimoto H, Sode K, Toyo'oka T, Todoroki K, Ikebukuro K.
Biosens Bioelectron. 2022 May 1;203:114027. doi: 10.1016/j.bios.2022.114027. Epub 2022 Jan 22.
2021年度
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Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands
Hasegawa H, Sasaki I, Tsukakoshi K, Ma Y, Nagasawa K, Numata S, Inoue Y, Kim Y, Ikebukuro K.
Int J Mol Sci. 2021 Dec 6;22(23):13159. doi: 10.3390/ijms222313159.
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Cytotoxic Aβ Protofilaments Are Generated in the Process of Aβ Fibril Disaggregation
Kaku T, Tsukakoshi K, Ikebukuro K.
Int J Mol Sci. 2021 Nov 26;22(23):12780. doi: 10.3390/ijms222312780.
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Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparallel Guanine-Quadruplex Structural Scaffold
Safitri FA, Tu ATT, Hoshi K, Shobo M, Zhao D, Witarto AB, Sumarsono SH, Giri-Rachman EA, Tsukakoshi K, Ikebukuro K, Yamazaki T.
Biomolecules. 2021 Nov 1;11(11):1617. doi: 10.3390/biom11111617.
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G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol
Tsukakoshi K, Yamagishi Y, Kanazashi M, Nakama K, Oshikawa D, Savory N, Matsugami A, Hayashi F, Lee J, Saito T, Sode K, Khunathai K, Kuno H, Ikebukuro K.
Nucleic Acids Res. 2021 Jun 21;49(11):6069-6081. doi: 10.1093/nar/gkab388.
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Data on G-quadruplex topology, and binding ability of G-quadruplex forming sequences found in the promoter region of biomarker proteins and those relations to the presence of nuclear localization signal in the proteins
Lee J, Teramoto K, Yokoyama T, Ueno K, Tsukakoshi K, Sode K, Ikebukuro K.
Data Brief. 2021 Apr 1;36:107028. doi: 10.1016/j.dib.2021.107028. eCollection 2021 Jun.
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G-quadruplex: Flexible conformational changes by cations, pH, crowding and its applications to biosensing
Nishio M, Tsukakoshi K, Ikebukuro K.
Biosens Bioelectron. 2021 Apr 15;178:113030. doi: 10.1016/j.bios.2021.113030. Epub 2021 Jan 23.
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A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria
Shono C, Ariyanti D, Abe K, Sakai Y, Sakamoto I, Tsukakoshi K, Sode K, Ikebukuro K.
Mar Biotechnol (NY). 2021 Feb;23(1):31-38. doi: 10.1007/s10126-020-09997-w. Epub 2020 Sep 26.
～2020年度
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Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF
Lee J, Tatsumi A, Tsukakoshi K, Wilson ED, Abe K, Sode K, Ikebukuro K.
Sensors (Basel). 2020 Jul 11;20(14):3878. doi: 10.3390/s20143878.
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Generation of C5-desoxy analogs of tetrahydroisoquinoline alkaloids exhibiting potent DNA alkylating ability
Tanifuji R, Tsukakoshi K, Ikebukuro K, Oikawa H, Oguri H.
Bioorg Med Chem Lett. 2019 Jul 15;29(14):1807-1811. doi: 10.1016/j.bmcl.2019.05.009. Epub 2019 May 9.
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G-Quadruplex Structure Improves the Immunostimulatory Effects of CpG Oligonucleotides
Hoshi K, Yamazaki T, Sugiyama Y, Tsukakoshi K, Tsugawa W, Sode K, Ikebukuro K.
Nucleic Acid Ther. 2019 Aug;29(4):224-229. doi: 10.1089/nat.2018.0761. Epub 2019 Mar 5.
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Anti-Idiotype DNA Aptamer Affinity Purification⁻High-Temperature Reversed-Phase Liquid Chromatography: A Simple, Accurate, and Selective Bioanalysis of Bevacizumab
Yamada T, Saito T, Shimizu Y, Tsukakoshi K, Hayashi H, Mizuno H, Tsuji D, Yamamoto K, Itoh K, Toyo'oka T, Ikebukuro K, Todoroki K.
Molecules. 2019 Feb 28;24(5):857. doi: 10.3390/molecules24050857.
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High-Throughput Bioanalysis of Bevacizumab in Human Plasma Based on Enzyme-Linked Aptamer Assay Using Anti-Idiotype DNA Aptamer
Yamada T, Saito T, Hill Y, Shimizu Y, Tsukakoshi K, Mizuno H, Hayashi H, Ikebukuro K, Toyo'oka T, Todoroki K.
Anal Chem. 2019 Feb 19;91(4):3125-3130. doi: 10.1021/acs.analchem.8b05725. Epub 2019 Feb 5.
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Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
Nishio M, Yamagishi A, Tsukakoshi K, Kato Y, Nakamura C, Ikebukuro K.
Methods Mol Biol. 2018;1867:165-174. doi: 10.1007/978-1-4939-8799-3_12.
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Esterification of PQQ Enhances Blood-Brain Barrier Permeability and Inhibitory Activity against Amyloidogenic Protein Fibril Formation
Tsukakoshi K, Yoshida W, Kobayashi M, Kobayashi N, Kim J, Kaku T, Iguchi T, Nagasawa K, Asano R, Ikebukuro K, Sode K.
ACS Chem Neurosci. 2018 Dec 19;9(12):2898-2903. doi: 10.1021/acschemneuro.8b00355. Epub 2018 Aug 8.
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Riboregulator elements as tools to engineer gene expression in cyanobacteria
Ueno K, Tsukakoshi K, Ikebukuro K.
Appl Microbiol Biotechnol. 2018 Sep;102(18):7717-7723. doi: 10.1007/s00253-018-9221-0. Epub 2018 Jul 13.
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CpG Methylation Changes G-Quadruplex Structures Derived from Gene Promoters and Interaction with VEGF and SP1
Tsukakoshi K, Saito S, Yoshida W, Goto S, Ikebukuro K.
Molecules. 2018 Apr 18;23(4):944. doi: 10.3390/molecules23040944.
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Improving the induction fold of riboregulators for cyanobacteria
Sakamoto I, Abe K, Kawai S, Tsukakoshi K, Sakai Y, Sode K, Ikebukuro K.
RNA Biol. 2018 Mar 4;15(3):353-358. doi: 10.1080/15476286.2017.1422470. Epub 2018 Feb 1.
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Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803
Ueno K, Sakai Y, Shono C, Sakamoto I, Tsukakoshi K, Hihara Y, Sode K, Ikebukuro K.
Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8465-8474. doi: 10.1007/s00253-017-8570-4. Epub 2017 Oct 16.
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Development of aptamers against unpurified proteins
Goto S, Tsukakoshi K, Ikebukuro K.
Biotechnol Bioeng. 2017 Dec;114(12):2706-2716. doi: 10.1002/bit.26389. Epub 2017 Aug 17.
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Development of HGF-binding aptamers with the combination of G4 promoter-derived aptamer selection and in silico maturation
Yokoyama T, Tsukakoshi K, Yoshida W, Saito T, Teramoto K, Savory N, Abe K, Ikebukuro K.
Biotechnol Bioeng. 2017 Oct;114(10):2196-2203. doi: 10.1002/bit.26354. Epub 2017 Jul 11.
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DNA aptamers against FokI nuclease domain for genome editing applications
Nishio M, Matsumoto D, Kato Y, Abe K, Lee J, Tsukakoshi K, Yamagishi A, Nakamura C, Ikebukuro K.
Biosens Bioelectron. 2017 Jul 15;93:26-31. doi: 10.1016/j.bios.2016.11.042. Epub 2016 Nov 22.
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Structural regulation by a G-quadruplex ligand increases binding abilities of G-quadruplex-forming aptamers
Tsukakoshi K, Ikuta Y, Abe K, Yoshida W, Iida K, Ma Y, Nagasawa K, Sode K, Ikebukuro K.
Chem Commun (Camb). 2016 Oct 18;52(85):12646-12649. doi: 10.1039/c6cc07552e.
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Improvement of the VEGF binding ability of DNA aptamers through in silico maturation and multimerization strategy
Fukaya T, Abe K, Savory N, Tsukakoshi K, Yoshida W, Ferri S, Sode K, Ikebukuro K.
J Biotechnol. 2015 Oct 20;212:99-105. doi: 10.1016/j.jbiotec.2015.08.011. Epub 2015 Aug 21.
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Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor development
Savory N, Takahashi Y, Tsukakoshi K, Hasegawa H, Takase M, Abe K, Yoshida W, Ferri S, Kumazawa S, Sode K, Ikebukuro K.
Biotechnol Bioeng. 2014 Mar;111(3):454-61. doi: 10.1002/bit.25111. Epub 2013 Sep 25.
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In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilis
Savory N, Lednor D, Tsukakoshi K, Abe K, Yoshida W, Ferri S, Jones BV, Ikebukuro K.
Biotechnol Bioeng. 2013 Oct;110(10):2573-80. doi: 10.1002/bit.24922. Epub 2013 Apr 22.
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Selection of DNA aptamers that recognize α-synuclein oligomers using a competitive screening method
Tsukakoshi K, Abe K, Sode K, Ikebukuro K.
Anal Chem. 2012 Jul 3;84(13):5542-7. doi: 10.1021/ac300330g. Epub 2012 Jun 12.
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Non-label homogeneous protein detection based on laser interferometric photo-thermal displacement measurement using aptamers
Tsukakoshi K, Ogasawara D, Takahashi E, Katayama R, Ikebukuro K.
Biotechnol J. 2011 Jan;6(1):101-6. doi: 10.1002/biot.201000158.
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Screening of DNA aptamer which binds to alpha-synuclein
Tsukakoshi K, Harada R, Sode K, Ikebukuro K.
Biotechnol Lett. 2010 May;32(5):643-8. doi: 10.1007/s10529-010-0200-5. Epub 2010 Jan 29.